Product Info Summary
SKU: | PB9503 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-alpha Sarcoglycan/SGCA Antibody Picoband™
View all alpha-Sarcoglycan Antibodies
SKU/Catalog Number
PB9503
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-alpha Sarcoglycan/SGCA Antibody Picoband™ catalog # PB9503. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-alpha Sarcoglycan/SGCA Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9503)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SGCA recombinant protein (Position: Q24-H387). Human SGCA shares 90.7% amino acid (aa) sequence identity with mouse SGCA.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9503 is reactive to SGCA in Human, Mouse, Rat
Applications
PB9503 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
43 kDa
Calculated molecular weight
42.875kDa
Background of alpha-Sarcoglycan
Alpha-sarcoglycan is a protein that in humans is encoded by the SGCA gene. This gene encodes a component of the dystrophin-glycoprotein complex (DGC), which is critical to the stability of muscle fiber membranes and to the linking of the actin cytoskeleton to the extracellular matrix. Its expression is thought to be restricted to striated muscle. Mutations in this gene result in type 2D autosomal recessive limb-girdle muscular dystrophy. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SGCA using anti-SGCA antibody (PB9503).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug,
Lane 2: Mouse Skeletal Muscle Tissue Lysate at 50ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGCA antigen affinity purified polyclonal antibody (Catalog # PB9503) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGCA at approximately 43 kDa. The expected band size for SGCA is at 43 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SGCA (Source: Uniprot.org, NCBI)
Gene Name
SGCA
Full Name
Alpha-sarcoglycan
Weight
42.875kDa
Superfamily
sarcoglycan alpha/epsilon family
Alternative Names
50-DAG; Adhalin; ADL; alphaSarcoglycan; alpha-Sarcoglycan; DAG2; DMDA2; DMDA250kD DAG; Dystroglycan-2; LGMD2D; LGMD2Dalpha (50kD dystrophin-associated glycoprotein); sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein); SCARMD1; SGCA SGCA 50DAG, ADL, DAG2, DMDA2, LGMD2D, LGMDR3, SCARMD1, adhalin sarcoglycan alpha alpha-sarcoglycan|50 kDa dystrophin-associated glycoprotein|50kD DAG|alpha-SG|dystroglycan-2|limb girdle muscular dystrophy 2D|sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SGCA, check out the SGCA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SGCA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-alpha Sarcoglycan/SGCA Antibody Picoband™ (PB9503)
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No publications found for PB9503
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-alpha Sarcoglycan/SGCA Antibody Picoband™
Question
We are currently using anti-alpha Sarcoglycan/SGCA antibody PB9503 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-29
Answer
The anti-alpha Sarcoglycan/SGCA antibody (PB9503) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-29