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Product Info Summary
| SKU: | A12601 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Siglec11 Antibody
SKU/Catalog Number
A12601
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Siglec11 Antibody (Catalog # A12601). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
Siglec11 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Siglec11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12601)
Host
Rabbit
Contents
Siglec11 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Siglec11 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human Siglec11. The immunogen is located within the last 50 amino acids of Siglec11.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of N4BP1 are known to exist; this antibody will only detect the longest isoform.
Reactive Species
A12601 is reactive to SIGLEC11 in Human
Applications
A12601 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
75.795kDa
Background of Siglec-11
Siglecs are sialic acid-binding lectins of the immunoglobulin superfamily that are mainly expressed in cells of the hematopoietic system. Siglec11 is unlike other siglecs in that it binds specifically to alpha2-8-linked sialic acids and is not found in peripheral blood leukocytes but instead on macrophages of various tissues. Siglec11 is highly homologous to Siglec10 over their extracellular domain but not over their cytoplasmic domain, suggesting that Siglec11 arose through gene duplication followed by a recombination event involving another ancestral siglec gene. Following treatment of Siglec11-transfected cells with pervanadate, Siglec11 becomes tyrosine-phosphorylated and strongly associates with SHP-1 and SHP-2. At least five isoforms of Siglec11 are known to exist. Siglec11 antibody will not cross-react with Siglec10.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Siglec11 antibody can be used for detection of Siglec11 by Western blot at 1 μg/mL. Despite its predicted size, Siglec11 migrates at a higher than expected molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Siglec11 in HepG2 cell lysate with Siglec11 antibody at 1 μg/mL.
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Immunohistochemistry of Siglec11 in human liver tissue with Siglec11 antibody at 5 μg/mL.
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Immunofluorescence of SIGLEC11 in human liver tissue with SIGLEC11 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For SIGLEC11 (Source: Uniprot.org, NCBI)
Gene Name
SIGLEC11
Full Name
Sialic acid-binding Ig-like lectin 11
Weight
75.795kDa
Superfamily
immunoglobulin superfamily
Alternative Names
sialic acid binding Ig-like lectin 11; sialic acid-binding Ig-like lectin 11; Sialic acid-binding lectin 11; Siglec11; Siglec-11 SIGLEC11 sialic acid binding Ig like lectin 11 sialic acid-binding Ig-like lectin 11|siglec-11
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SIGLEC11, check out the SIGLEC11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SIGLEC11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Siglec11 Antibody (A12601)
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