Product Info Summary
SKU: | A08437 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-SIRPB1/Cd172B Antibody
SKU/Catalog Number
A08437
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-SIRPB1/Cd172B Antibody (Catalog # A08437). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SIRPB1/Cd172B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08437)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human SIRPB1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08437 is reactive to SIRPB1 in Human
Applications
A08437 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
43.211kDa
Background of SIRPB1
Immunoglobulin-like cell surface receptor involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Participates also in the recruitment of tyrosine kinase SYK.
Kharitonenkov A., Nature 386:181-186(1997).
Ota T., Nat. Genet. 36:40-45(2004).
Deloukas P., Nature 414:865-871(2001).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HuvEc cells, COLO cells and HeLa cells, using SIRPB1 antibody A08437.
Protein Target Info & Infographic
Gene/Protein Information For SIRPB1 (Source: Uniprot.org, NCBI)
Gene Name
SIRPB1
Full Name
Signal-regulatory protein beta-1
Weight
43.211kDa
Alternative Names
SIRP beta 1/gamma SIRPB1 CD172b, SIRP-BETA-1 signal regulatory protein beta 1 signal-regulatory protein beta-1|CD172 antigen-like family member B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SIRPB1, check out the SIRPB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SIRPB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SIRPB1/Cd172B Antibody (A08437)
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No publications found for A08437
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-SIRPB1/Cd172B Antibody
Question
My question regarding product A08437, anti-SIRPB1/Cd172B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08437 anti-SIRPB1/Cd172B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-05-01
Question
Would A08437 anti-SIRPB1/Cd172B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
You can see on the product datasheet, A08437 anti-SIRPB1/Cd172B antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-31
Question
We are currently using anti-SIRPB1/Cd172B antibody A08437 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
The anti-SIRPB1/Cd172B antibody (A08437) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-22
Question
We need to test anti-SIRPB1/Cd172B antibody A08437 on human skin for research purposes, then I may be interested in using anti-SIRPB1/Cd172B antibody A08437 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
F. Jackson
Verified customer
Asked: 2014-06-18
Answer
The products we sell, including anti-SIRPB1/Cd172B antibody A08437, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-06-18