Anti-SIRPB1/Cd172B Antibody

SIRPB1 antibody

Boster Bio Anti-SIRPB1/Cd172B Antibody (Catalog # A08437). Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A08437
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-SIRPB1/Cd172B Antibody

View all SIRPB1 Antibodies

SKU/Catalog Number

A08437

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-SIRPB1/Cd172B Antibody (Catalog # A08437). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SIRPB1/Cd172B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08437)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human SIRPB1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08437 is reactive to SIRPB1 in Human

Applications

A08437 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

43.211kDa

Background of SIRPB1

Immunoglobulin-like cell surface receptor involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Participates also in the recruitment of tyrosine kinase SYK.

Kharitonenkov A., Nature 386:181-186(1997).
Ota T., Nat. Genet. 36:40-45(2004).
Deloukas P., Nature 414:865-871(2001).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For SIRPB1 (Source: Uniprot.org, NCBI)

Gene Name

SIRPB1

Full Name

Signal-regulatory protein beta-1

Weight

43.211kDa

Alternative Names

SIRP beta 1/gamma SIRPB1 CD172b, SIRP-BETA-1 signal regulatory protein beta 1 signal-regulatory protein beta-1|CD172 antigen-like family member B

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SIRPB1, check out the SIRPB1 Infographic

SIRPB1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SIRPB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-SIRPB1/Cd172B Antibody

Question

My question regarding product A08437, anti-SIRPB1/Cd172B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-05-01

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08437 anti-SIRPB1/Cd172B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-05-01

Question

Would A08437 anti-SIRPB1/Cd172B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-31

Answer

You can see on the product datasheet, A08437 anti-SIRPB1/Cd172B antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-31

Question

We are currently using anti-SIRPB1/Cd172B antibody A08437 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-22

Answer

The anti-SIRPB1/Cd172B antibody (A08437) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-22

Question

We need to test anti-SIRPB1/Cd172B antibody A08437 on human skin for research purposes, then I may be interested in using anti-SIRPB1/Cd172B antibody A08437 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

F. Jackson

Verified customer

Asked: 2014-06-18

Answer

The products we sell, including anti-SIRPB1/Cd172B antibody A08437, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2014-06-18

Order DetailsPrice
A08437-100uL

100uL

$360

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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