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Product Info Summary
| SKU: | A03854 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-SkiP SNW1 Antibody
SKU/Catalog Number
A03854
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SkiP SNW1 Antibody (Catalog # A03854). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SkiP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SkiP SNW1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03854)
Host
Rabbit
Contents
SkiP Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SkiP antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human SkiP. The immunogen is located within the last 50 amino acids of SkiP.
Reactive Species
A03854 is reactive to SNW1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
61.5 kDa
Background of SNW1
TGF-beta and the bone morphogenic proteins (BMPs) are key signaling proteins that regulate numerous cellular processes such as embryonic development and tumorigenesis. Both signal through the Smad protein family and are negatively regulated by Ski and SnoN, two related proto-oncoproteins. Ski functions by binding to the Smad proteins activated by TGF-beta and the (BMPs) and preventing their phosphorylation, inhibiting their ability to bind DNA and activate the transcription of downstream genes. SkiP was originally identified as a Ski-interacting protein and was later found to augment the signals induced by TGF-beta but inhibit transcription induced by BMP-2 in C2C12 cells, suggesting that SkiP is a key player in the signaling cascades inititated by TGF-beta and the BMP protein family.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03854 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SkiP antibody can be used for detection of SkiP by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SkiP in mouse skeletal muscle tissue lysate with SkiP antibody at 0.5 μg/ml.
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Immunofluorescence of Ski in human kidney tissue with Ski antibody at 20 μg/mL.
Specific Publications For Anti-SkiP SNW1 Antibody (A03854)
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