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Product Info Summary
| SKU: | A00544 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SKP2 Antibody Picoband™
SKU/Catalog Number
A00544
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SKP2 Antibody Picoband™ catalog # A00544. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SKP2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00544)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SKP2, which shares 88.9% and 94.4% amino acid (aa) sequence identity with mouse and rat SKP2, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00544 is reactive to SKP2 in Human, Mouse, Rat
Applications
A00544 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
48 kDa
Calculated molecular weight
47.761kDa
Background of SKP2
The F box protein Skp2 (S-phase kinase-associated protein 2) is oncogenic, and its frequent amplification and overexpression correlate with the grade of malignancy of certain tumors. Skp2 controls p300-p53 signaling pathways in cancer cells, making it a potential molecular target for cancer therapy. This gene positively regulates the G (1)-S transition by controlling the stability of several G (1) regulators, such as the cell cycle inhibitor p27. This study provides evidence of a role for an F-box protein in oncogenesis and establishes SKP2 as a protooncogene causally involved in the pathogenesis of lymphomas.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SKP2 using anti-SKP2 antibody (A00544).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human SGC-7901 whole cell lysates,
Lane 2: human SK-OV-3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SKP2 antigen affinity purified polyclonal antibody (Catalog # A00544) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SKP2 at approximately 48KD. The expected band size for SKP2 is at 48KD.
Protein Target Info & Infographic
Gene/Protein Information For SKP2 (Source: Uniprot.org, NCBI)
Gene Name
SKP2
Full Name
S-phase kinase-associated protein 2
Weight
47.761kDa
Alternative Names
CDK2/cyclin A-associated protein p45; cyclin A/CDK2-associated protein p45; Cyclin-A/CDK2-associated protein p45; FBL1; F-box protein Skp2; F-box/LRR-repeat protein 1; FBXL1; FBXL1MGC1366; FLB1; p45skp2; Skp2; S-phase kinase-associated protein 2 (p45); S-phase kinase-associated protein 2 SKP2 FBL1, FBXL1, FLB1, p45 S-phase kinase associated protein 2 S-phase kinase-associated protein 2|CDK2/cyclin A-associated protein p45|F-box/LRR-repeat protein 1|S-phase kinase-associated protein 2, E3 ubiquitin protein ligase|p45skp2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SKP2, check out the SKP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SKP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SKP2 Antibody Picoband™ (A00544)
Hello CJ!
A00544 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xu F, Zhu X, Han T, You X, Liu F, Ye L, Zhang X, Wang X, Yao Y. Int J Oncol. 2014 Jul;45(1):255-63. Doi: 10.3892/Ijo.2014.2411. Epub 2014 Apr 29. The Oncoprotein Hepatitis B X-Interacting Protein Promotes The Migration Of Ovarian Cancer Cells Thro...
Su Y, Wang F, Qi H, Zhao Sg, Li X, Cui H. Mol Vis. 2011 Jan 22;17:247-56. Small Interfering Rna Targeting Of S Phase Kinase-Interacting Protein 2 Inhibits Cell Proliferation Of Pterygium Fibroblasts.
Chen Xm, Xie Xb, Zhao Q, Wang F, Bai Y, Yin Jq, Jiang H, Xie Xl, Jia Q, Huang G. Mol Med Rep. 2015 Jan;11(1):105-12. Doi: 10.3892/Mmr.2014.2733. Epub 2014 Oct 21. Ampelopsin Induces Apoptosis By Regulating Multiple C-Myc/S-Phase Kinase-Associated ...
Chen Xm, Bai Y, Zhong Yj, Xie Xl, Long Hw, Yang Yy, Wu Sg, Jia Q, Wang Xh. Plos One. 2013 Nov 12;8(11):E79201. Doi: 10.1371/Journal.Pone.0079201. Ecollection 2013. Wogonin Has Multiple Anti-Cancer Effects By Regulating C-Myc/Skp2/Fbw7?? And Hdac1/...
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