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- Table of Contents
SLC25A6 antibody (A09457-2) performs well in ELISA, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of SLC25A6 using anti-SLC25A6 antibody (A09457-2).
SLC25A6 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-SLC25A6 Antibody (A09457-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
SLC25A6 was detected in a paraffin-embedded section of human kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-SLC25A6 Antibody (A09457-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-SLC25A6 Antibody
SLC25A6 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | ELISA, IP, IF, IHC, ICC, WB |
Tested Applications
SLC25A6 antibody was positively detected by WB in:
rat heart tissue lysates, mouse heart tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SLC25A6 antibody was positively detected by IHC in:
uman kidney tissue, mouse heart tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
1:500-2000
Immunohistochemistry
1:50-400
Immunocytochemistry/Immunofluorescence
1:50-400
ImmunoPrecipitation
1:250-300
ELISA
1:100-1000
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
IP
ELISA
Rat
Tested
Mouse
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Human
Recommend
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-SLC25A6 Antibody
View all SLC25A6 Antibodies
View all SLC25A6 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
Peptide
Observed Molecular Weight
33 kDa
Calculated Molecular Weight
32.9 kDa
Background of SLC25A6
SLC25A6, also known as ANT3 (adenine nucleotide translocase 3), is a member of the solute carrier family 25, specifically the mitochondrial carrier subfamily. As part of the mitochondrial carrier family, SLC25A6 plays a role in the mitochondrial energy production process by facilitating the exchange of metabolites necessary for oxidative phosphorylation (PMID: 23506884).
Cite This Product
Anti-SLC25A6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09457-2)
Protocols
Western Blot protocol for SLC25A6 antibody
Immunohistochemistry (IHC) protocol for SLC25A6 antibody
Review
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Product Promise
We promise Anti-SLC25A6 Antibody performs excellently in relevant applications for ELISA, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A09457-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026