Anti-SLC6A18 Antibody Picoband™

Product Name

Anti-SLC6A18 Antibody Picoband™

View all Slc6a18 Antibodies

SKU/Catalog Number

A10812-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SLC6A18 Antibody Picoband™ catalog # A10812-1. Tested in ELISA, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SLC6A18 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10812-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SLC6A18 recombinant protein (Position: M1-A569).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A10812-1 is reactive to Slc6a18 in Human, Rat

Applications

A10812-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

71 kDa

Calculated molecular weight

69.229kDa

Background of Slc6a18

Solute carrier family 6, member 18 also known as SLC6A18 is a protein which in humans is encoded by the SLC6A18 gene. The SLC6 family of proteins, which includes SLC6A18, act as specific transporters for neurotransmitters, amino acids, and osmolytes like betaine, taurine, and creatine. SLC6 proteins are sodium cotransporters that derive the energy for solute transport from the electrochemical gradient for sodium ions.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Rat
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SLC6A18 using anti-SLC6A18 antibody (A10812-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde
r reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: rat kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC6A18 antigen affinity purified polyclonal antibody (Catalog # A10812-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC6A18 at approximately 71 kDa. The expected band size for SLC6A18 is at 71 kDa.

Gene/Protein Information For Slc6a18 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ31236; Sodium- and chloride-dependent transporter XTRP2; sodium channel-like protein; sodium-dependent neutral amino acid transporter B(0)AT3; solute carrier family 6 (neurotransmitter transporter), member 18; Solute carrier family 6 member 18; solute carrier family 6, member 18; System B(0) neutral amino acid transporter AT3; XTRP2 Slc6a18|B0AT3, D630001K16Rik, Xt2, Xtr, Xtrp2|solute carrier family 6 (neurotransmitter transporter), member 18|sodium-dependent neutral amino acid transporter B(0)AT3|X transporter protein 2|b(0)AT3|sodium and chloride dependent transporter Xtrp2|sodium- and chloride-dependent transporter XTRP2|solute carrier family 6 member 18|system B 0 neutral amino acid transporter AT3

For more info on Slc6a18, check out the Slc6a18 Infographic

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In this infographic, you will see the following information for Slc6a18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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