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- Table of Contents
DIABLO Antibodies
AND DIABLO ELISA kits, DIABLO Proteins
Many biological assays use antibodies to detect DIABLO, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with DIABLO in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop DIABLO antibodies...
Designing an IHC experiment for DIABLO? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the DIABLO IHC GuideDIABLO Infographic by Boster Bio
All DIABLO Products
Product
Figures
Specificity
Applications
Price
Human
ELISA
$500
Cat # EZ0838
For 5 plates, 96 wells each
Cat # EZ0838
For 5 plates, 96 wells each
Anti-Smac/Diablo Antibody Picoband®
1 Citations
Human, Mouse, Rat
ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB
$370
Cat # PB9860
100 μg/vial
Cat # PB9860
100 μg/vial
Human
ELISA
$499
Cat # EK0838
96 wells/kit, with removable strips.
Cat # EK0838
96 wells/kit, with removable strips.
Human
ELISA
$499
Cat # FEK0838
96 wells/kit, with removable strips.
Cat # FEK0838
96 wells/kit, with removable strips.
Anti-Smac/Diablo Rabbit Monoclonal Antibody
1 Citations
Human, Mouse, Rat
Flow Cytometry, IP, IF, IHC, ICC, WB
$370
Cat # M03790
100 μl
Cat # M03790
100 μl
DIABLO Overview
Facts about Diablo homolog, mitochondrial.
Diablo homolog, mitochondrial (DIABLO)
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP).
Inhibits the action of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis- inhibiting action of XIAP/BIRC4 by boosting XIAP/BIRC4 ubiquitination and degradation during the ubiquitin-proteasome pathway.
Gene Information
| Human | |
|---|---|
| Gene Name: | DIABLO |
| Uniprot: | Q9NR28 |
| Entrez: | 56616 |
Family
| Belongs to: |
|---|
| No superfamily |
Alternative Names
0610041G12Rik; Diablo; diablo, IAP-binding mitochondrial protein; Direct IAP-binding protein with low pI; mitochondrial Smac protein; Second mitochondria-derived activator of caspase; SMAC; SMACmitochondrial
Molecular Weight
Mass (kDA):
27.131 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 12q24.31 |
| Sequence: | 12; NC_000012.12 (122207662..122227456, complement) |
Tissue Specificity
Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed.
Subcellular Localizations
Mitochondrion. Released into the cytosol when cells undergo apoptosis.
Diseases
- Malignant Neoplasms
- Neoplasms
- Carcinoma
- Leukemia
- Malignant Paraganglionic Neoplasm
- Malignant Neoplasm Of Prostate
- Lung Neoplasms
- Liver Carcinoma
- Melanoma
- Malignant Neoplasm Of Breast
- Malignant Tumor Of Colon
- Adenocarcinoma
Interacting Proteins
- ARL6IP1
- BIRC2
- BIRC5
- BIRC7
- PRKCD
- XIAP
Pathways
- Cell Death
- Induction Of Apoptosis
- Cell Cycle
- Programmed Cell Death
- Sensitization
- Cell Growth
- Cell Proliferation
- Localization
- Pathogenesis
- Mitochondrial Outer Membrane Permeabilization
- Rna Interference
- Proteolysis
- Mitochondrial Translocation
PTMs
- Cleavage
- Phosphorylation
- Ubiquitination
- Dephosphorylation
- Oxidation
- Acetylation
- Reduction
Research Areas
- Apoptosis
- Cancer
- Cell Biology
- Mitochondrial Markers
- Tumor Suppressors
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/DIABLO
bosterbio.com/bosterbio-gene-info-cards/DIABLO
References
PMID: 10929711 by Du C., et al. Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition.
PMID: 10950947 by Srinivasula S.M., et al. Molecular determinants of the caspase-promoting activity of Smac/DIABLO and its role in the death receptor pathway.
1 Publications of Boster DIABLO Antibodies & ELISA Kits
- PubMed ID: 28081713, You F, Li Q, Jin G, Zheng Y, Chen J, Yang H. BMC Neurosci. 2017 Jan 12;18(1):12....
*More publications can be found for each product on its corresponding product page