Click image to see more details
Product Info Summary
| SKU: | M00728-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Smad1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00728-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Smad1 Rabbit Monoclonal Antibody catalog # M00728-1. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Smad1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00728-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CAC-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Smad1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00728-1 is reactive to SMAD1 in Human, Mouse
Applications
M00728-1 is guaranteed for IF, ICC, WB Boster Guarantee
Observed Molecular Weight
90 kDa
Calculated molecular weight
52.26kDa
Background of SMAD1
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Western blot analysis of Smad1 expression in HeLa cell lysate.
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Immunohistochemical analysis of paraffin-embedded human kidney, using Smad1 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For SMAD1 (Source: Uniprot.org, NCBI)
Gene Name
SMAD1
Full Name
Mothers against decapentaplegic homolog 1
Weight
52.26kDa
Superfamily
dwarfin/SMAD family
Alternative Names
BSP1; BSP-1; hSMAD1; JV4-1SMAD, mothers against DPP homolog 1 (Drosophila); MAD homolog 1; MADH1JV41; MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila); Mad-related protein 1; mothers against decapentaplegic homolog 1; Mothers against DPP homolog 1; SMAD family member 1SMAD 1; SMAD, mothers against DPP homolog 1; Smad1; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1 SMAD1 BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1 SMAD family member 1 mothers against decapentaplegic homolog 1|MAD, mothers against decapentaplegic homolog 1|Mad-related protein 1|SMAD, mothers against DPP homolog 1|TGF-beta signaling protein 1|mothers against DPP homolog 1|transforming growth factor-beta signaling protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMAD1, check out the SMAD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMAD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Smad1 Rabbit Monoclonal Antibody (M00728-1)
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No publications found for M00728-1
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Smad1 Rabbit Monoclonal Antibody
Question
Will anti-Smad1 Rabbit Monoclonal antibody M00728-1 work for IHC with ovarian carcinoma?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
According to the expression profile of ovarian carcinoma, SMAD1 is highly expressed in ovarian carcinoma. So, it is likely that anti-Smad1 Rabbit Monoclonal antibody M00728-1 will work for IHC with ovarian carcinoma.
Boster Scientific Support
Answered: 2020-04-16
Question
I see that the anti-Smad1 Rabbit Monoclonal antibody M00728-1 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-07
Question
Can you help my question with product M00728-1, anti-Smad1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00728-1 anti-Smad1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-27
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ovarian carcinoma using anti-Smad1 Rabbit Monoclonal antibody M00728-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-28
Question
We are currently using anti-Smad1 Rabbit Monoclonal antibody M00728-1 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
The anti-Smad1 Rabbit Monoclonal antibody (M00728-1) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-17
Question
We ordered your anti-Smad1 Rabbit Monoclonal antibody for IHC on uterus last year. I am using mouse, and I plan to use the antibody for IF next. We need examining uterus as well as ovarian carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
S. Krishna
Verified customer
Asked: 2019-10-08
Answer
I have checked the website and datasheets of our anti-Smad1 Rabbit Monoclonal antibody and it appears that M00728-1 has been validated on mouse in both IHC and IF. Thus M00728-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-08
Question
Here is the WB image, lot number and protocol we used for ovarian carcinoma using anti-Smad1 Rabbit Monoclonal antibody M00728-1. Please let me know if you require anything else.
B. Miller
Verified customer
Asked: 2019-08-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-29
Question
I was wanting to use your anti-Smad1 Rabbit Monoclonal antibody for IHC for human ovarian carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human ovarian carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
You can see on the product datasheet, M00728-1 anti-Smad1 Rabbit Monoclonal antibody has been tested for IF, IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human ovarian carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-09
Question
I am looking for using your anti-Smad1 Rabbit Monoclonal antibody for ub-specific processing proteases studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
J. Jones
Verified customer
Asked: 2019-04-17
Answer
I appreciate your inquiry. This M00728-1 anti-Smad1 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IF, IHC, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-04-17
Question
My team were happy with the WB result of your anti-Smad1 Rabbit Monoclonal antibody. However we have been able to see positive staining in kidney cytoplasm. using this antibody. Is that expected? Could you tell me where is SMAD1 supposed to be expressed?
L. Anderson
Verified customer
Asked: 2018-11-19
Answer
From what I have seen in literature, kidney does express SMAD1. Generally SMAD1 expresses in cytoplasm. Regarding which tissues have SMAD1 expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 8637600
Mammary carcinoma, Pubmed ID: 8663601
Ovarian carcinoma, Pubmed ID: 9136927
Placenta, Pubmed ID: 8774881, 15489334
Uterus, Pubmed ID: 14702039, 17974005
Boster Scientific Support
Answered: 2018-11-19
Question
I am interested in to test anti-Smad1 Rabbit Monoclonal antibody M00728-1 on human ovarian carcinoma for research purposes, then I may be interested in using anti-Smad1 Rabbit Monoclonal antibody M00728-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-04-25
Answer
The products we sell, including anti-Smad1 Rabbit Monoclonal antibody M00728-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-04-25
Question
We have observed staining in mouse uterus. Any tips? Is anti-Smad1 Rabbit Monoclonal antibody supposed to stain uterus positively?
Verified Customer
Verified customer
Asked: 2017-07-10
Answer
According to literature uterus does express SMAD1. According to Uniprot.org, SMAD1 is expressed in visceral pleura, kidney, mammary carcinoma, placenta, uterus, ovarian carcinoma, among other tissues. Regarding which tissues have SMAD1 expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 8637600
Mammary carcinoma, Pubmed ID: 8663601
Ovarian carcinoma, Pubmed ID: 9136927
Placenta, Pubmed ID: 8774881, 15489334
Uterus, Pubmed ID: 14702039, 17974005
Boster Scientific Support
Answered: 2017-07-10
Question
Is this M00728-1 anti-Smad1 Rabbit Monoclonal antibody reactive to the isotypes of SMAD1?
G. Miller
Verified customer
Asked: 2016-07-19
Answer
The immunogen of M00728-1 anti-Smad1 Rabbit Monoclonal antibody is A synthesized peptide derived from human Smad1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-07-19
Question
Is a blocking peptide available for product anti-Smad1 Rabbit Monoclonal antibody (M00728-1)?
P. Anderson
Verified customer
Asked: 2013-10-02
Answer
We do provide the blocking peptide for product anti-Smad1 Rabbit Monoclonal antibody (M00728-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-10-02
Question
Is there a BSA free version of anti-Smad1 Rabbit Monoclonal antibody M00728-1 available?
L. Williams
Verified customer
Asked: 2013-04-26
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Smad1 Rabbit Monoclonal antibody M00728-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-04-26
Question
Would M00728-1 anti-Smad1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Miller
Verified customer
Asked: 2013-01-21
Answer
As indicated on the product datasheet, M00728-1 anti-Smad1 Rabbit Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-01-21
