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- Table of Contents
SMAD2 antibody (M00090-1) performs well in Flow Cytometry, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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Immunofluorescent analysis of Hela cells, using Smad2 Antibody.
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Anti-Smad2 Rabbit Monoclonal Antibody
SMAD2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, ICC, WB |
Tested Applications
SMAD2 antibody was positively detected by WB in:
human Jurkat whole cell, human Hela whole cell, human MCF-7 whole cell, human MDA-MB-453 whole cell, rat C6 whole cell, mouse RAW2647 whole cell, human Hela- WT whole cell lysates, human Hela-SMAD2 KO whole cell lysates
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SMAD2 antibody was positively detected by ICC/IF in:
Hela cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
ICC/IF 1:50-200
FC 1:20
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
FC
Human
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Product Information
Product Name
Anti-Smad2 Rabbit Monoclonal Antibody
View all SMAD2 Antibodies
View all SMAD2 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACI-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Smad2
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
52 kDa
Calculated Molecular Weight
52.3 kDa
Cite This Product
Anti-Smad2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00090-1)
Protocols
Western Blot protocol for SMAD2 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for SMAD2 antibody
Publications
Application:
Species:
Rod-Shaped Microgel Scaffolds with Interconnective Pores and Oxygen-generating Functions Promote Skin Wound Healing and Alleviate Hypertrophic Scar Formation
Published: 2025-01-16
Journal: ADVANCED FUNCTIONAL MATERIALS
Application: WB
Impact Factor: 19.00
Species: Mouse
A platform of functional studies of ESCC-associated gene mutations identifies the roles of TGFBR2 in ESCC progression and metastasis
Published: 2024-11-10
Journal: Cell Reports
Application: WB
Impact Factor: 7.50
Species: Mouse
PMID: 39527477
Riboflavin protects against pancreatic cancer metastasis by targeting TGF-β receptor 1
Published: 2024-03-16
Journal: BIOORGANIC CHEMISTRY
Application: WB,IHC
Impact Factor: 4.50
Species: Human
PMID: 38503026
Berberine Attenuates Cigarette Smoke Extract-induced Airway Inflammation in Mice: Involvement of TGF-β1/Smads Signaling Pathway
Published: 2019-10-14
Journal: Current Medical Science
Application: WB
Impact Factor: 0.00
Species: Mouse
PMID: 31612392
Review
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Product Promise
We promise Anti-Smad2 Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00090-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026