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Product Info Summary
| SKU: | A02823 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-SMURF1 Antibody
SKU/Catalog Number
A02823
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SMURF1 Antibody (Catalog # A02823). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SMURF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-SMURF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02823)
Host
Rabbit
Contents
SMURF1 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SMURF1 antibody was raised against a 16 amino acid peptide near the amino terminus of human SMURF1. The immunogen is located within amino acids 180 - 230 of SMURF1.
Cross-reactivity
SMURF1 antibody is human, mouse and rat reactive. At least three isoforms of SMURF1 are known to exist this antibody will recognize all three isoforms. SMURF1 is predicted to not cross-react with SMURF2.
Reactive Species
A02823 is reactive to SMURF1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
86.1 kDa
Background of SMURF1
SMURF1 (SMAD ubiquitin regulatory factor 1) is a negative regulator of TGF-beta signaling (1). SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1,2). SMURF1 negatively regulates osteoblast activity and response to bone morphogenesis protein (BMP) through controlling MEKK2 degradation (3). SMURF1 has recently been shown to play a role in breast cancer cell migration and invasion through the downregulation of RhoA (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02823 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SMURF1 body can be used for detection of SMURF1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SMURF1 in 293 cell lysate with SMURF1 antibody at 1 μg/ml.
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Immunohistochemistry of SMURF1 in rat kidney tissue with SMURF1 antibody at 5 μg/ml.
Specific Publications For Anti-SMURF1 Antibody (A02823)
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