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- Table of Contents
SMURF1 antibody (A02823) performs well in ELISA, IHC-P, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunohistochemistry of SMURF1 in rat kidney tissue with SMURF1 antibody at 5 μg/ml.
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Anti-SMURF1 Antibody
SMURF1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
SMURF1 body can be used for detection of SMURF1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-SMURF1 Antibody
View all SMURF1 Antibodies
View all SMURF1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
SMURF1 antibody can be stored at 4°C, stable for up to one year.
Host
Rabbit
Contents
SMURF1 antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SMURF1 antibody was raised against a 16 amino acid peptide near the amino terminus of human SMURF1. The immunogen is located within amino acids 180 - 230 of SMURF1.
Cross-Reactivity
SMURF1 antibody is human, mouse and rat reactive. At least three isoforms of SMURF1 are known to exist this antibody will recognize all three isoforms. SMURF1 is predicted to not cross-react with SMURF2.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
86.1 kDa
Background of SMURF1
SMURF1 (SMAD ubiquitin regulatory factor 1) is a negative regulator of TGF-beta signaling (1). SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1,2). SMURF1 negatively regulates osteoblast activity and response to bone morphogenesis protein (BMP) through controlling MEKK2 degradation (3). SMURF1 has recently been shown to play a role in breast cancer cell migration and invasion through the downregulation of RhoA (4).
Cite This Product
Anti-SMURF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02823)
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Product Promise
We promise Anti-SMURF1 Antibody performs excellently in relevant applications for ELISA, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A02823
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026