Click image to see more details
Product Info Summary
| SKU: | A04281 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SMYD2/Kmt3C Antibody
SKU/Catalog Number
A04281
Size
100μl
Form
Liquid
Description
Boster Bio Anti-SMYD2/Kmt3C Antibody catalog # A04281. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SMYD2/Kmt3C Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04281)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
5G7
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human SMYD2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04281 is reactive to SMYD2 in Human, Mouse, Rat
Applications
A04281 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
49.688kDa
Background of SMYD2
Cysteine string protein (CSP) is a 34 kDa rat brain protein localized to the cytoplasmic surface of the synaptic vesicle. CSP is believed to perform similar functions in the assembly of synaptic vesicle proteins which are essential to membrane trafficking.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of specific cells using SMYD2 Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For SMYD2 (Source: Uniprot.org, NCBI)
Gene Name
SMYD2
Full Name
N-lysine methyltransferase SMYD2
Weight
49.688kDa
Superfamily
class V-like SAM-binding methyltransferase superfamily
Alternative Names
EC 2.1.1.43; Histone methyltransferase SMYD2; HSKM-Bzinc finger, MYND domain containing 14; KMT3CEC 2.1.1.-; Lysine N-methyltransferase 3C; MGC119305; SET and MYND domain containing 2; SET and MYND domain-containing protein 2; ZMYND14 SMYD2 HSKM-B, KMT3C, ZMYND14 SET and MYND domain containing 2 N-lysine methyltransferase SMYD2|SET and MYND domain-containing protein 2|histone methyltransferase SMYD2|lysine N-methyltransferase 3C|zinc finger, MYND domain containing 14
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMYD2, check out the SMYD2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMYD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SMYD2/Kmt3C Antibody (A04281)
Hello CJ!
No publications found for A04281
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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13 Customer Q&As for Anti-SMYD2/Kmt3C Antibody
Question
We are currently using anti-SMYD2/Kmt3C antibody A04281 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
The anti-SMYD2/Kmt3C antibody (A04281) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-13
Question
Is a blocking peptide available for product anti-SMYD2/Kmt3C antibody (A04281)?
K. Dhar
Verified customer
Asked: 2019-11-11
Answer
We do provide the blocking peptide for product anti-SMYD2/Kmt3C antibody (A04281). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-11
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-SMYD2/Kmt3C antibody A04281. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-14
Question
Does A04281 anti-SMYD2/Kmt3C antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
As indicated on the product datasheet, A04281 anti-SMYD2/Kmt3C antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-12
Question
I see that the anti-SMYD2/Kmt3C antibody A04281 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-05
Question
I have attached the WB image, lot number and protocol we used for testis using anti-SMYD2/Kmt3C antibody A04281. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-05
Question
I would like to test anti-SMYD2/Kmt3C antibody A04281 on human testis for research purposes, then I may be interested in using anti-SMYD2/Kmt3C antibody A04281 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Singh
Verified customer
Asked: 2019-05-20
Answer
The products we sell, including anti-SMYD2/Kmt3C antibody A04281, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-20
Question
Does anti-SMYD2/Kmt3C antibody A04281 work on horse WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2018-07-17
Answer
Our lab technicians have not tested anti-SMYD2/Kmt3C antibody A04281 on horse. You can run a BLAST between horse and the immunogen sequence of anti-SMYD2/Kmt3C antibody A04281 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-17
Question
Is this A04281 anti-SMYD2/Kmt3C antibody reactive to the isotypes of SMYD2?
Verified Customer
Verified customer
Asked: 2017-10-06
Answer
The immunogen of A04281 anti-SMYD2/Kmt3C antibody is Synthesized peptide derived from the Internal region of human SMYD2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-06
Question
Would anti-SMYD2/Kmt3C antibody A04281 work for WB with testis?
Z. Banerjee
Verified customer
Asked: 2017-09-21
Answer
According to the expression profile of testis, SMYD2 is highly expressed in testis. So, it is likely that anti-SMYD2/Kmt3C antibody A04281 will work for WB with testis.
Boster Scientific Support
Answered: 2017-09-21
Question
I have a question about product A04281, anti-SMYD2/Kmt3C antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Miller
Verified customer
Asked: 2015-07-01
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04281 anti-SMYD2/Kmt3C antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-07-01
Question
Do you have a BSA free version of anti-SMYD2/Kmt3C antibody A04281 available?
R. Li
Verified customer
Asked: 2013-07-01
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SMYD2/Kmt3C antibody A04281 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-07-01
Question
I was wanting to use your anti-SMYD2/Kmt3C antibody for WB for human testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human testis identification?
R. Collins
Verified customer
Asked: 2013-05-17
Answer
You can see on the product datasheet, A04281 anti-SMYD2/Kmt3C antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-05-17
