Anti-SMYD2/Kmt3C Antibody

Boster Bio Anti-SMYD2/Kmt3C Antibody catalog # A04281. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A04281
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-SMYD2/Kmt3C Antibody

See all SMYD2 products

SKU/Catalog Number

A04281

Size

100μl

Form

Liquid

Description

Boster Bio Anti-SMYD2/Kmt3C Antibody catalog # A04281. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SMYD2/Kmt3C Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04281)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human SMYD2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04281 is reactive to SMYD2 in Human, Mouse, Rat

Applications

A04281 is guaranteed for WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For SMYD2 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9NRG4

Gene ID

56950

Gene Name

SMYD2

Full Name

N-lysine methyltransferase SMYD2

Weight

49.688kDa

Superfamily

class V-like SAM-binding methyltransferase superfamily

Alternative Names

EC 2.1.1.43; Histone methyltransferase SMYD2; HSKM-Bzinc finger, MYND domain containing 14; KMT3CEC 2.1.1.-; Lysine N-methyltransferase 3C; MGC119305; SET and MYND domain containing 2; SET and MYND domain-containing protein 2; ZMYND14 SMYD2 HSKM-B, KMT3C, ZMYND14 SET and MYND domain containing 2 N-lysine methyltransferase SMYD2|SET and MYND domain-containing protein 2|histone methyltransferase SMYD2|lysine N-methyltransferase 3C|zinc finger, MYND domain containing 14

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SMYD2, check out the SMYD2 Infographic

SMYD2 infographic

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In this infographic you will see the following information for SMYD2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for A04281

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13 Customer Q&As for Anti-SMYD2/Kmt3C Antibody

Question

We are currently using anti-SMYD2/Kmt3C antibody A04281 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-13

Answer

The anti-SMYD2/Kmt3C antibody (A04281) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-13

Question

Is a blocking peptide available for product anti-SMYD2/Kmt3C antibody (A04281)?

K. Dhar

Verified customer

Asked: 2019-11-11

Answer

We do provide the blocking peptide for product anti-SMYD2/Kmt3C antibody (A04281). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-11-11

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-SMYD2/Kmt3C antibody A04281. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-08-14

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-14

Question

Does A04281 anti-SMYD2/Kmt3C antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-07-12

Answer

As indicated on the product datasheet, A04281 anti-SMYD2/Kmt3C antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-07-12

Question

I see that the anti-SMYD2/Kmt3C antibody A04281 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-05

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-05

Question

I have attached the WB image, lot number and protocol we used for testis using anti-SMYD2/Kmt3C antibody A04281. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-06-05

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-05

Question

I would like to test anti-SMYD2/Kmt3C antibody A04281 on human testis for research purposes, then I may be interested in using anti-SMYD2/Kmt3C antibody A04281 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

D. Singh

Verified customer

Asked: 2019-05-20

Answer

The products we sell, including anti-SMYD2/Kmt3C antibody A04281, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-05-20

Question

Does anti-SMYD2/Kmt3C antibody A04281 work on horse WB with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2018-07-17

Answer

Our lab technicians have not tested anti-SMYD2/Kmt3C antibody A04281 on horse. You can run a BLAST between horse and the immunogen sequence of anti-SMYD2/Kmt3C antibody A04281 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse cervix carcinoma in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-07-17

Question

Is this A04281 anti-SMYD2/Kmt3C antibody reactive to the isotypes of SMYD2?

Verified Customer

Verified customer

Asked: 2017-10-06

Answer

The immunogen of A04281 anti-SMYD2/Kmt3C antibody is Synthesized peptide derived from the Internal region of human SMYD2. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-10-06

Question

Would anti-SMYD2/Kmt3C antibody A04281 work for WB with testis?

Z. Banerjee

Verified customer

Asked: 2017-09-21

Answer

According to the expression profile of testis, SMYD2 is highly expressed in testis. So, it is likely that anti-SMYD2/Kmt3C antibody A04281 will work for WB with testis.

Boster Scientific Support

Answered: 2017-09-21

Question

I have a question about product A04281, anti-SMYD2/Kmt3C antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

B. Miller

Verified customer

Asked: 2015-07-01

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04281 anti-SMYD2/Kmt3C antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2015-07-01

Question

Do you have a BSA free version of anti-SMYD2/Kmt3C antibody A04281 available?

R. Li

Verified customer

Asked: 2013-07-01

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SMYD2/Kmt3C antibody A04281 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2013-07-01

Question

I was wanting to use your anti-SMYD2/Kmt3C antibody for WB for human testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human testis identification?

R. Collins

Verified customer

Asked: 2013-05-17

Answer

You can see on the product datasheet, A04281 anti-SMYD2/Kmt3C antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-05-17

Size

Total: $299

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A04281
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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