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Product Info Summary
| SKU: | M33970 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-SOD2 Antibody
SKU/Catalog Number
M33970
Size
80 µl
Description
Boster Bio Anti-SOD2 Antibody (Catalog # M33970). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-SOD2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M33970)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
37CT127.5.11.6
Isotype
IgG1κ
Immunogen
Purified His-tagged SOD protein (Fragment) was used to produced this monoclonal antibody.
Reactive Species
M33970 is reactive to SOD2 in Human
Calculated molecular weight
24.8 kDa
Background of SOD2
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M33970 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:10-1:50
Validation Images & Assay Conditions
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Western blot analysis of anti-SOD2 Monoclonal Antibody by SOD2-GST fusion protein (GST MW=26 kD. SOD2-GST fusion protein (arrow) was detected using the purified Mab. (1:2000)
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All lanes : Anti-SD Antibody at 1:2000 dilution
Lane 1: Hela whole cell lysates
Lane 2: human brain lysates
Lane 3: human heart lysates
Lane 4: human liver lysates
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 25 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOD2 Monoclonal Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Specific Publications For Anti-SOD2 Antibody (M33970)
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