Product Info Summary
SKU: | A10698-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Solo/SESTD1 Antibody Picoband™
SKU/Catalog Number
A10698-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Solo/SESTD1 Antibody Picoband™ catalog # A10698-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Solo/SESTD1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10698-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Solo/SESTD1 recombinant protein (Position: Q549-E690).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10698-1 is reactive to SESTD1 in Human, Mouse, Rat
Applications
A10698-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
79 kDa
Calculated molecular weight
79.348kDa
Background of Solo
SEC14 and spectrin domains 1, also known as SEC14 domain and spectrin repeat-containing protein 1 and Solo, is a protein that in humans is encoded by the SESTD1 gene. Enables phosphatidylinositol-3,4-bisphosphate binding activity and phospholipid binding activity. Involved in negative regulation of calcium ion transmembrane transport via high voltage-gated calcium channel. Located in intermediate filament cytoskeleton. Colocalizes with calcium channel complex.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry, 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Solo/SESTD1 using anti-Solo/SESTD1 antibody (A10698-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat testis tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Solo/SESTD1 antigen affinity purified polyclonal antibody (Catalog # A10698-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Solo/SESTD1 at approximately 79 kDa. The expected band size for Solo/SESTD1 is at 79 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of Daudi cells using anti-Solo/SESTD1 antibody (A10698-1).
Overlay histogram showing Daudi cells stained with A10698-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Solo/SESTD1 Antibody (A10698-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For SESTD1 (Source: Uniprot.org, NCBI)
Gene Name
SESTD1
Full Name
SEC14 domain and spectrin repeat-containing protein 1
Weight
79.348kDa
Superfamily
SOLO family
Alternative Names
DKFZp434O0515; Huntingtin-interacting protein-like protein; Protein Solo; SEC14 and spectrin domains 1; SEC14 domain and spectrin repeat-containing protein 1; SOLO SESTD1 SOLO SEC14 and spectrin domain containing 1 SEC14 domain and spectrin repeat-containing protein 1|SEC14 and spectrin domains 1|huntingtin-interacting protein-like protein|protein Solo
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SESTD1, check out the SESTD1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SESTD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Solo/SESTD1 Antibody Picoband™ (A10698-1)
Hello CJ!
No publications found for A10698-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Solo/SESTD1 Antibody Picoband™?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Solo/SESTD1 Antibody Picoband™
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question