Product Info Summary
SKU: | A00058 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Sonic hedgehog protein SHH Antibody
View all Sonic Hedgehog/Shh Antibodies
SKU/Catalog Number
A00058
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Sonic hedgehog protein SHH Antibody catalog # A00058. Tested in WB,ICC/IF,IHC,Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Sonic hedgehog protein SHH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00058)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human ERalpha around the phosphorylation site of S118. Sequence: 108-128aa
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00058 is reactive to SHH in Human
Applications
A00058 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
49.607kDa
Background of Sonic Hedgehog/Shh
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. ATP-Binding Cassette Sub-Family B Member 1 (ABCB1, also named P-glycoprotein) is a plasma membrane-associated multidrug transporter that utilizes the energy of ATP hydrolysis to pump toxic xenobiotics out of cells. Unique features of ABCB1 are its very broad substrate specificity and its basal ATPase activity in the absence of transport substrates. Human ABCB1 plays an important role in absorption, distribution, metabolism, excretion and toxicity of pharmacologically relevant drugs. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anti-cancer drugs. This protein also functions as a transporter across the blood-brain barrier.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:2,000
ICC: 1:50-1:200
IHC: 1:200-1:500
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
ICC staining Sonic Hedgehog Protein in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Click image to see more details
Western blot analysis of Sonic Hedgehog Protein on different lysates using anti-Sonic Hedgehog Protein antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2
Protein Target Info & Infographic
Gene/Protein Information For SHH (Source: Uniprot.org, NCBI)
Gene Name
SHH
Full Name
Sonic hedgehog protein
Weight
49.607kDa
Superfamily
hedgehog family
Alternative Names
HHG1; HHG-1; HLP3; HPE3; MCOPCB5; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; ShhNC; SMMCI; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS SHH HHG1, HLP3, HPE3, MCOPCB5, SMMCI, ShhNC, TPT, TPTPS sonic hedgehog signaling molecule sonic hedgehog protein|shh unprocessed N-terminal signaling and C-terminal autoprocessing domains|sonic hedgehog homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SHH, check out the SHH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SHH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Sonic hedgehog protein SHH Antibody (A00058)
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No publications found for A00058
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Recommended Resources
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