Anti-Smad4(DPC4) Antibody (Monoclonal, DCS-46)

Boster Bio Anti-Smad4 (DPC4) Antibody (Monoclonal, DCS-46) catalog # MA1089. Tested in ICC, WB applications. This antibody reacts with Human. Cited in 6 publication(s).

Product Info Summary

SKU: MA1089
Size: 100μg/vial
Reactive Species: Human
Host: Mouse
Application: ICC, WB

Product Name

Anti-Smad4(DPC4) Antibody (Monoclonal, DCS-46)

See all Smad4 products

SKU/Catalog Number

MA1089

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Smad4 (DPC4) Antibody (Monoclonal, DCS-46) catalog # MA1089. Tested in ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Smad4(DPC4) Antibody (Monoclonal, DCS-46) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1089)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

DCS-46

Isotype

Mouse IgG1

Immunogen

Recombinant human Smad4 (DPC4).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1089 is reactive to Smad4 in Human

Applications

MA1089 is guaranteed for ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunocytochemistry , 1μg/ml, Human, -
Western blot, 2-4μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For Smad4 (Source: Uniprot.Org, NCBI)

Uniprot ID

O70437

Gene ID

50554

Gene Name

Smad4

Full Name

Mothers against decapentaplegic homolog 4

Weight

60.469kDa

Superfamily

dwarfin/SMAD family

Alternative Names

DPC4; DPC4SMAD, mothers against DPP homolog 4 (Drosophila); hSMAD4; JIP; MAD homolog 4; MADH4; MADH4mothers against decapentaplegic homolog 4; mothers against decapentaplegic homolog 4; mothers against decapentaplegic, Drosophila, homolog of, 4; SMAD 4; SMAD family member 4MAD, mothers against decapentaplegic homolog 4 (Drosophila); SMAD, mothers against DPP homolog 4; Smad4 Smad4|Madh4|SMAD family member 4|mothers against decapentaplegic homolog 4|MAD (mothers against decapentaplegic Drosophila) homolog 4|MAD homolog 4|SMAD 4|mothers against DPP homolog 4

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Smad4, check out the Smad4 Infographic

Smad4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Smad4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1089 has been cited in 6 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

TGF%u2010%u03B2 regulates the proliferation of lung adenocarcinoma cells by inhibiting PIK3R3 expression

Emodin protects rat liver from CCl4-induced fibrogenesis via inhibition of hepatic stellate cells activation

Effect of Kuijie Granule on the Expression of TGF-?/Smads Signaling Pathway in Patients with Ulcerative Colitis

Molecular responses of radiation-induced liver damage in rats

Chen Wh, Kong Xy, Wan R, Xiao Cs, Li L, Wang Zy, Lin N, Wang Hm. Chin J Integr Med. 2012 May;18(5):378-84. Doi: 10.1007/S11655-012-1086-Y. Epub 2012 May 2. Effects Of Huogu I Formula (I) On Correlated Factors Of Bone Regeneration In Chickens With ...

Tang Y, Li Y, Yu H, Gao C, Liu L, Chen S, Xing M, Liu L, Yao P. J Nutr Biochem. 2014 Jun;25(6):675-82. Doi: 10.1016/J.Jnutbio.2014.02.009. Epub 2014 Mar 19. Quercetin Prevents Ethanol-Induced Iron Overload By Regulating Hepcidin Through The Bmp6/S...

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16 Customer Q&As for Anti-Smad4(DPC4) Antibody (Monoclonal, DCS-46)

Question

We have seen staining in human kidney. Do you have any suggestions? Is anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) supposed to stain kidney positively?

Verified Customer

Verified customer

Asked: 2020-04-22

Answer

From what I have seen in literature kidney does express SMAD4. From what I have seen in Uniprot.org, SMAD4 is expressed in kidney, fetal brain, placenta, muscle, liver, among other tissues. Regarding which tissues have SMAD4 expression, here are a few articles citing expression in various tissues:
Fetal brain, Pubmed ID: 8553070
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 15489334
Placenta, Pubmed ID: 8774881

Boster Scientific Support

Answered: 2020-04-22

Question

I have attached the WB image, lot number and protocol we used for fetal brain using anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) MA1089. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-03

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-03

Question

My team were satisfied with the WB result of your anti-Smad4(DPC4) antibody (Monoclonal, DCS-46). However we have observed positive staining in muscle cytoplasm using this antibody. Is that expected? Could you tell me where is SMAD4 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-01-21

Answer

According to literature, muscle does express SMAD4. Generally SMAD4 expresses in cytoplasm. Regarding which tissues have SMAD4 expression, here are a few articles citing expression in various tissues:
Fetal brain, Pubmed ID: 8553070
Liver, Pubmed ID: 24275569
Muscle, Pubmed ID: 15489334
Placenta, Pubmed ID: 8774881

Boster Scientific Support

Answered: 2020-01-21

Question

Is there a BSA free version of anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) MA1089 available?

Verified Customer

Verified customer

Asked: 2019-10-09

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) MA1089 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-10-09

Question

My question regarding product MA1089, anti-Smad4(DPC4) antibody (Monoclonal, DCS-46). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-09-23

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1089 anti-Smad4(DPC4) antibody (Monoclonal, DCS-46), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-09-23

Question

Will MA1089 anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-06

Answer

As indicated on the product datasheet, MA1089 anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-06

Question

Is this MA1089 anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) reactive to the isotypes of SMAD4?

Verified Customer

Verified customer

Asked: 2019-09-03

Answer

The immunogen of MA1089 anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) is Recombinant human Smad4(DPC4). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-09-03

Question

Is a blocking peptide available for product anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) (MA1089)?

T. Moore

Verified customer

Asked: 2019-08-02

Answer

We do provide the blocking peptide for product anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) (MA1089). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-08-02

Question

We have tried in the past anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) for WB on muscle in the past. I am using human, and We intend to use the antibody for ICC next. I am interested in examining muscle as well as kidney in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?

Verified Customer

Verified customer

Asked: 2019-07-30

Answer

I looked at the website and datasheets of our anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) and it appears that MA1089 has been validated on human in both WB and ICC. Thus MA1089 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-07-30

Question

We want using your anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) for r-rno-2173796; smad2/smad3:smad4 heterotrimer regulates transcription studies. Has this antibody been tested with western blotting on hepg2 whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-03-14

Answer

Thanks for your inquiry. This MA1089 anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) is tested on human hek293 whole cell lysate, hepg2 whole cell lysate, rat c6 whole cell lysate. It is guaranteed to work for ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-03-14

Question

I was wanting to use your anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) for ICC for human fetal brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human fetal brain identification?

F. Thomas

Verified customer

Asked: 2018-11-19

Answer

As indicated on the product datasheet, MA1089 anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) has been tested for ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human fetal brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-11-19

Question

My lab would like to test anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) MA1089 on human fetal brain for research purposes, then I may be interested in using anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) MA1089 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-08-28

Answer

The products we sell, including anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) MA1089, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-08-28

Question

I see that the anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) MA1089 works with ICC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-03-01

Answer

You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-03-01

Question

Will anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) MA1089 work for ICC with fetal brain?

Verified Customer

Verified customer

Asked: 2017-06-14

Answer

According to the expression profile of fetal brain, SMAD4 is highly expressed in fetal brain. So, it is likely that anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) MA1089 will work for ICC with fetal brain.

Boster Scientific Support

Answered: 2017-06-14

Question

We are currently using anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) MA1089 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?

K. Jha

Verified customer

Asked: 2014-02-11

Answer

The anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) (MA1089) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-02-11

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain using anti-Smad4(DPC4) antibody (Monoclonal, DCS-46) MA1089. Let me know if you need anything else.

N. Banerjee

Verified customer

Asked: 2013-04-03

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-04-03

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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