radio
SKU MA1089
Size 100μg/vial
Reactivity Human
Clonality Monoclonal
Clone Number DCS-46
Host Mouse
Ig Isotype Mouse IgG1
Applications ICC, WB

Overview

Product Name Anti-Smad4(DPC4) Antibody (Monoclonal, DCS-46)
SKU/Catalog Number MA1089
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for Smad4 (DPC4), SMAD family member 4 (SMAD4) detection. Tested with WB, ICC in Human. No cross reactivity with other proteins.
Cite This Product Anti-Smad4(DPC4) Antibody (Monoclonal, DCS-46) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1089)
Host Mouse
Immunogen Recombinant human Smad4(DPC4).
Reactivity Human

Assay Details

Assay Dilutions Overview

Immunocytochemistry , 1μg/ml, Human, -
Western blot, 2-4μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.

Images And Assay Conditions

/antibody/ma1089 1 WB anti smad4 dpc4 antibody monoclonal dcs 46.jpg

Anti-Smad4(DPC4) antibody (monoclonal), MA1089, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id O70437
Gene Name Smad4
Protein Name Mothers against decapentaplegic homolog 4
Alternative Names Mothers against decapentaplegic homolog 4;MAD homolog 4;Mothers against DPP homolog 4;SMAD family member 4;SMAD 4;Smad4;Smad4;Madh4;
Subcellular Localization Cytoplasm . Nucleus . In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with R-SMAD. PDPK1 prevents its nuclear translocation. .
Molecular Weight 60469 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC- 3') within BMP response element (BMPRE) of cardiac activating regions. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8 (By similarity). .
Background SMAD4 plays a pivotal role in signal transduction of the transforming growth factor beta superfamily cytokines by mediating transcriptional activation of target genes. Smad4 signalling in T cells is required for suppression of gastrointestinal cancer. Mutational inactivation of SMAD4 causes TGF-beta unresponsiveness and gave a basis for understanding the physiologic role of this gene in tumorigenesis. Mutations in DPC4(SMAD4) cause juvenile polyposis syndrome, but only account for a minority of cases.

Order Product (MA1089)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $240
100ug+Free HRP Secondary BA1050 $240
100ug+Free Biotin Secondary BA1001 $240
Ships in 5-7 business days.

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Publications

Emodin protects rat liver from CCl4-induced fibrogenesis via inhibition of hepatic stellate cells activation
Effect of Kuijie Granule on the Expression of TGF-?/Smads Signaling Pathway in Patients with Ulcerative Colitis
Molecular responses of radiation-induced liver damage in rats
Chen Wh, Kong Xy, Wan R, Xiao Cs, Li L, Wang Zy, Lin N, Wang Hm. Chin J Integr Med. 2012 May;18(5):378-84. Doi: 10.1007/S11655-012-1086-Y. Epub 2012 May 2. Effects Of Huogu I Formula (I) On Correlated Factors Of Bone Regeneration In Chickens With ...
Tang Y, Li Y, Yu H, Gao C, Liu L, Chen S, Xing M, Liu L, Yao P. J Nutr Biochem. 2014 Jun;25(6):675-82. Doi: 10.1016/J.Jnutbio.2014.02.009. Epub 2014 Mar 19. Quercetin Prevents Ethanol-Induced Iron Overload By Regulating Hepcidin Through The Bmp6/S...

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to smad4 antibody, dpc4 antibody