Data & Images
|Product Name||Anti-Smad4(DPC4) Antibody (Monoclonal, DCS-46)|
|Description||Mouse IgG monoclonal antibody for Smad4 (DPC4), SMAD family member 4 (SMAD4) detection. Tested with WB, ICC in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-Smad4(DPC4) Antibody (Monoclonal, DCS-46) DCS-46 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1089)|
|Replacement Item||This antibody may replace the following items: sc-7966|sc-56479|sc-56775|sc-1908|sc-7154|sc-73599|sc-1909|sc-73040|sc-1909-R|sc-7966-X|sc-1908-X|sc-7154-X|sc-1909-X from Santa Cruz Biotechnology.|
|Predicted Species||Bovine, Monkey|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||Recombinant human Smad4(DPC4).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Mothers against decapentaplegic homolog 4|
|Molecular Weight||60469 MW|
|Protein Function||Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC- 3') within BMP response element (BMPRE) of cardiac activating regions. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8 (By similarity). .|
|Sequence Similarities||Belongs to the dwarfin/SMAD family.|
|Subcellular Localization||Cytoplasm . Nucleus . In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with R-SMAD. PDPK1 prevents its nuclear translocation. .|
|Alternative Names||Mothers against decapentaplegic homolog 4;MAD homolog 4;Mothers against DPP homolog 4;SMAD family member 4;SMAD 4;Smad4;Smad4;Madh4;|
|Research Areas|||signal transduction|signaling pathway|nuclear signaling|smads| epigenetics and nuclear signaling|nuclear signaling pathways| stem cells|tgf beta|cytoplasmic| cancer|oncoproteins/suppressors|tumor suppressors| cardiovascular|heart|apoptosis|cardiogenesis|transcription factors/regulators|hypertrophy|transcription factors|vasculature|endothelium|cancer metabolism|response to hypoxia| metabolism|pathways and processes|metabolism processes||
Background for Mothers against decapentaplegic homolog 4
Anti-Smad4(DPC4) Antibody (Monoclonal, DCS-46) Images
Click the images to enlarge.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate