Product Info Summary
SKU: | PA2284 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Transcription factor SOX-2 SOX2 Antibody
SKU/Catalog Number
PA2284
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Transcription factor SOX-2 SOX2 Antibody catalog # PA2284. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Transcription factor SOX-2 SOX2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2284)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human SOX2, different from the related mouse and rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2284 is reactive to SOX2 in Human, Mouse, Rat
Applications
PA2284 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
36 kDa
Calculated molecular weight
34.31kDa
Background of SOX2
SRY (sex determining region Y)-box 2, also known as SOX2, is a transcription factor that is essential for maintaining self-renewal, or pluripotency of undifferentiated embryonic stem cells. Sox2 is a member of the Sox family of transcription factors, which have been shown to play key roles in many stages of mammalian development. This gene is mapped to 3q26.33. It is found that SOX2 can regulate OCT3/4 expression and maintains ES pluripotency through upstream transcription factors. SOX2 is identified as a lineage-survival oncogene in lung and esophageal squamous cell carcinoma. In addition to those, SOX2 has a critical role in maintenance of embryonic and neural stem cells and holds great promise in research involving induced pluripotency, an emerging and very promising field of regenerative medicine.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of SOX2 using anti-SOX2 antibody (PA2284).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX2 antigen affinity purified polyclonal antibody (Catalog # PA2284) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX2 at approximately 36 kDa. The expected band size for SOX2 is at 34 kDa.
Click image to see more details
Figure 2. IHC analysis of SOX2 using anti-SOX2 antibody (PA2284).
SOX2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SOX2 Antibody (PA2284) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of SOX2 using anti-SOX2 antibody (PA2284).
SOX2 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SOX2 Antibody (PA2284) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of SOX2 using anti-SOX2 antibody (PA2284).
SOX2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-SOX2 Antibody (PA2284) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For SOX2 (Source: Uniprot.org, NCBI)
Gene Name
SOX2
Full Name
Transcription factor SOX-2
Weight
34.31kDa
Alternative Names
ANOP3; MCOPS3; MGC2413; SOX2; SRY (sex determining region Y)-box 2; SRY-related HMG-box gene 2; transcription factor SOX2; transcription factor SOX-2 SOX2 ANOP3, MCOPS3 SRY-box transcription factor 2 transcription factor SOX-2|SRY (sex determining region Y)-box 2|SRY-box 2|SRY-related HMG-box gene 2|sex determining region Y-box 2|transcription factor SOX2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SOX2, check out the SOX2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SOX2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Transcription factor SOX-2 SOX2 Antibody (PA2284)
Hello CJ!
PA2284 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yi H, Xie B, Liu B, Wang X, Xu L, Liu J, Li M, Zhong X, Peng F. Stem Cells Int. 2018 Jan 24;2018:3628578. doi: 10.1155/2018/3628578. eCollection 2018. Derivation and Identification of Motor Neurons from Human Urine-Derived Induced Pluripotent Stem...
Cheng Z, Wang Hz, Li X, Wu Z, Han Y, Li Y, Chen G, Xie X, Huang Y, Du Z, Zhou Y. J Exp Clin Cancer Res. 2015 Mar 26;34:27. Doi: 10.1186/S13046-015-0142-9. Microrna-184 Inhibits Cell Proliferation And Invasion, And Specifically Targets Tnfaip2 In G...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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17 Customer Q&As for Anti-Transcription factor SOX-2 SOX2 Antibody
Question
We have seen staining in rat retina. What should we do? Is anti-SOX2 antibody supposed to stain retina positively?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
According to literature retina does express SOX2. According to Uniprot.org, SOX2 is expressed in cerebral cortex, fetal brain, retina, lung, among other tissues. Regarding which tissues have SOX2 expression, here are a few articles citing expression in various tissues:
Fetal brain, Pubmed ID: 7849401
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-20
Question
Can you help my question with product PA2284, anti-SOX2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2284 anti-SOX2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-14
Question
I would like using your anti-SOX2 antibody for neuronal stem cell population maintenance studies. Has this antibody been tested with western blotting on brain tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
Thank you for your inquiry. This PA2284 anti-SOX2 antibody is tested on rat lung tissue, tissue lysate, mouse brain, brain tissue, cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-06
Question
Our team were well pleased with the WB result of your anti-SOX2 antibody. However we have been able to see positive staining in retina nucleus. using this antibody. Is that expected? Could you tell me where is SOX2 supposed to be expressed?
H. Anderson
Verified customer
Asked: 2019-11-18
Answer
According to literature, retina does express SOX2. Generally SOX2 expresses in nucleus. Regarding which tissues have SOX2 expression, here are a few articles citing expression in various tissues:
Fetal brain, Pubmed ID: 7849401
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-11-18
Question
Does anti-SOX2 antibody PA2284 work on pig IHC with retina?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
Our lab technicians have not tested anti-SOX2 antibody PA2284 on pig. You can run a BLAST between pig and the immunogen sequence of anti-SOX2 antibody PA2284 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig retina in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-20
Question
Would anti-SOX2 antibody PA2284 work for IHC with fetal brain?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
According to the expression profile of fetal brain, SOX2 is highly expressed in fetal brain. So, it is likely that anti-SOX2 antibody PA2284 will work for IHC with fetal brain.
Boster Scientific Support
Answered: 2019-07-09
Question
Is this PA2284 anti-SOX2 antibody reactive to the isotypes of SOX2?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
The immunogen of PA2284 anti-SOX2 antibody is A synthetic peptide corresponding to a sequence in the middle region of human SOX2(171-183aa YSMMQDQLGYPQH), different from the related mouse and rat sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-03
Question
I see that the anti-SOX2 antibody PA2284 works with IHC, what is the protocol used to produce the result images on the product page?
A. Johnson
Verified customer
Asked: 2019-02-15
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-02-15
Question
Would PA2284 anti-SOX2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-01-17
Answer
You can see on the product datasheet, PA2284 anti-SOX2 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-17
Question
Is a blocking peptide available for product anti-SOX2 antibody (PA2284)?
Verified Customer
Verified customer
Asked: 2019-01-07
Answer
We do provide the blocking peptide for product anti-SOX2 antibody (PA2284). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-01-07
Question
I was wanting to use your anti-SOX2 antibody for IHC for human fetal brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human fetal brain identification?
J. Anderson
Verified customer
Asked: 2018-10-10
Answer
You can see on the product datasheet, PA2284 anti-SOX2 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human fetal brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-10
Question
See below the WB image, lot number and protocol we used for fetal brain using anti-SOX2 antibody PA2284. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-09-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-24
Question
We ordered your anti-SOX2 antibody for IHC on lung in a previous project. I am using rat, and I plan to use the antibody for WB next. My lab would like examining lung as well as cerebral cortex in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-04-11
Answer
I have checked the website and datasheets of our anti-SOX2 antibody and it appears that PA2284 has been validated on rat in both IHC and WB. Thus PA2284 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-04-11
Question
I am looking for to test anti-SOX2 antibody PA2284 on human fetal brain for research purposes, then I may be interested in using anti-SOX2 antibody PA2284 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
V. Singh
Verified customer
Asked: 2017-11-09
Answer
The products we sell, including anti-SOX2 antibody PA2284, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-09
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain using anti-SOX2 antibody PA2284. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-06-21
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-21
Question
We are currently using anti-SOX2 antibody PA2284 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
B. Jones
Verified customer
Asked: 2016-09-21
Answer
The anti-SOX2 antibody (PA2284) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-09-21
Question
Is there a BSA free version of anti-SOX2 antibody PA2284 available?
B. Singh
Verified customer
Asked: 2016-04-06
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-SOX2 antibody PA2284 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-04-06