Product Info Summary
SKU: | A00177 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Sox9 (Q175) Antibody
SKU/Catalog Number
A00177
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Sox9 (Q175) Antibody catalog # A00177. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Sox9 (Q175) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00177)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human p38 around the phosphorylation site of Y323.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00177 is reactive to SOX9 in Human, Mouse, Rat
Applications
A00177 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
56.137kDa
Background of SOX9
Anti cdk2 antibody recognizes a protein encoded by a gene member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of SOX9 using Anti-Sox9 (Q175) Antibody (A00177).
Immunohistochemistry (IHC) analyzes of Sox9 (Q175) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-Sox9 (Q175) Antibody A00177
Western blot (WB) analysis of Sox9 (Q175) polyclonal antibody at 1:500 dilutionLane1:C6 whole cell lysate(40ug)
Lane2:BV2 whole cell lysate(40ug)
Lane3:Hela whole cell lysate(40ug)
Lane4:Panc1 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Click image to see more details
Figure 3. Western blotting validation for Anti-Sox9 (Q175) Antibody A00177
Western blot (WB) analysis of Sox9 (Q175) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate
Lane2:sp2/0 whole cell lysate
Lane3:PC12 whole cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For SOX9 (Source: Uniprot.org, NCBI)
Gene Name
SOX9
Full Name
Transcription factor SOX-9
Weight
56.137kDa
Alternative Names
campomelic dysplasia, autosomal sex-reversal; CMD 1; CMD1; CMPD1; SOX9; SRA1SRY (sex-determining region Y)-box 9 protein; SRY (sex determining region Y)-box 9; SRY-related HMG-box, gene 9; transcription factor SOX-9 SOX9 CMD1, CMPD1, SRA1, SRXX2, SRXY10 SRY-box transcription factor 9 transcription factor SOX-9|SRY (sex determining region Y)-box9|SRY (sex-determining region Y)-box 9 protein|SRY-box 9|SRY-related HMG-box, gene 9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SOX9, check out the SOX9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SOX9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Sox9 (Q175) Antibody (A00177)
Hello CJ!
No publications found for A00177
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Sox9 (Q175) Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Sox9 (Q175) Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question