MMP-3 Matrix Metalloproteinase-3 Human Recombinant Protein, HEK

MMP-3 protein, Human

MMP-3 Human Recombinant produced in HEK293 cells is a proform of the Human MMP3 [Tyr18-Cys477 (Lys45Glu)] and fused with a ployhistide tag at the C-terminus, having an Mw of 52kDa. MMP-3 is purified by proprietary chromatographic techniques.

Product Info Summary

SKU: PROTP08254
Size: 2ug, 10ug, 1mg
Origin Species: Human
Source: HEK293

Product Name

MMP-3 Matrix Metalloproteinase-3 Human Recombinant Protein, HEK

View all MMP-3 recombinant proteins

SKU/Catalog Number

PROTP08254

Size

2ug, 10ug, 1mg

Description

MMP-3 Human Recombinant produced in HEK293 cells is a proform of the Human MMP3 [Tyr18-Cys477 (Lys45Glu)] and fused with a ployhistide tag at the C-terminus, having an Mw of 52kDa. MMP-3 is purified by proprietary chromatographic techniques.

Storage & Handling

Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Cite This Product

MMP-3 Matrix Metalloproteinase-3 Human Recombinant Protein, HEK (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP08254)

Form

Sterile Filtered colorless solution.

Formulation

The MMP-3 is supplied as a 0.2µm filtered solution in 20mM Tris-HCl, 150mM NaCl and 0.05% Brij35, pH 7.5.

Purity

Greater than 95% as determined by SDS-PAGE.

Predicted MW

53.977kDa

Biological Activity

The activity was measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is greater than 150 pmoles/min/µg.
Recombinant Human MMP-3 protein pro form needs to be activated with Chymotrypsin.
Activation Protocol:
1. Dilute MMP3 to 20µg/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.
2. Activate MMP3 by adding Chymotrypsin(Sigma, Catalog#C­3142,1mg/ml stock in 1mM HCl) to a final concentration of 5ug/ml.
3. Incubate at 37°C for 30 minutes.
4. Stop activation with 2mM PMSF. Pre-warm the PMSF to 37°C prior to adding to sample.

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Validation Images & Assay Conditions

Gene/Protein Information For MMP3 (Source: Uniprot.org, NCBI)

Gene Name

MMP3

Full Name

Stromelysin-1

Weight

53.977kDa

Superfamily

peptidase M10A family

Alternative Names

CHDS6; EC 3.4.24; EC 3.4.24.17; matrix metallopeptidase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3 (stromelysin 1, progelatinase); Matrix metalloproteinase-3; MGC126102; MGC126103; MMP3; MMP-3; proteoglycanase; SL-1; STMY; STMY1MGC126104; STR1; Stromelysin 1; stromelysin-1; transin-1 MMP3 CHDS6, MMP-3, SL-1, STMY, STMY1, STR1 matrix metallopeptidase 3 stromelysin-1|matrix metalloproteinase 3 (stromelysin 1, progelatinase)|proteoglycanase|transin-1

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on MMP3, check out the MMP3 Infographic

MMP3 infographic

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In this infographic, you will see the following information for MMP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].

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SKU:PROTP08254

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