Product Info Summary
SKU: | PROTP08254 |
---|---|
Size: | 2ug, 10ug, 1mg |
Origin Species: | Human |
Source: | HEK293 |
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Product info
Product Name
MMP-3 Matrix Metalloproteinase-3 Human Recombinant Protein, HEK
View all MMP-3 recombinant proteins
SKU/Catalog Number
PROTP08254
Size
2ug, 10ug, 1mg
Description
MMP-3 Human Recombinant produced in HEK293 cells is a proform of the Human MMP3 [Tyr18-Cys477 (Lys45Glu)] and fused with a ployhistide tag at the C-terminus, having an Mw of 52kDa. MMP-3 is purified by proprietary chromatographic techniques.
Storage & Handling
Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Cite This Product
MMP-3 Matrix Metalloproteinase-3 Human Recombinant Protein, HEK (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP08254)
Form
Sterile Filtered colorless solution.
Formulation
The MMP-3 is supplied as a 0.2µm filtered solution in 20mM Tris-HCl, 150mM NaCl and 0.05% Brij35, pH 7.5.
Purity
Greater than 95% as determined by SDS-PAGE.
Predicted MW
53.977kDa
Biological Activity
The activity was measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is greater than 150 pmoles/min/µg.
Recombinant Human MMP-3 protein pro form needs to be activated with Chymotrypsin.
Activation Protocol:
1. Dilute MMP3 to 20µg/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.
2. Activate MMP3 by adding Chymotrypsin(Sigma, Catalog#C3142,1mg/ml stock in 1mM HCl) to a final concentration of 5ug/ml.
3. Incubate at 37°C for 30 minutes.
4. Stop activation with 2mM PMSF. Pre-warm the PMSF to 37°C prior to adding to sample.
Assay dilution & Images
Validation Images & Assay Conditions
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Recombinant protein fun image
Protein Target Info & Infographic
Gene/Protein Information For MMP3 (Source: Uniprot.org, NCBI)
Gene Name
MMP3
Full Name
Stromelysin-1
Weight
53.977kDa
Superfamily
peptidase M10A family
Alternative Names
Stromelysin-1; EC 3.4.24.17; Matrix metalloproteinase-3; MMP-3; Transin-1; SL-1; STMY; STR1; STMY1; MGC126102; MGC126103; MGC126104 MMP3 CHDS6, MMP-3, SL-1, STMY, STMY1, STR1 matrix metallopeptidase 3 stromelysin-1|matrix metalloproteinase 3 (stromelysin 1, progelatinase)|proteoglycanase|transin-1
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on MMP3, check out the MMP3 Infographic
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In this infographic, you will see the following information for MMP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].
Specific Publications For MMP-3 Matrix Metalloproteinase-3 Human Recombinant Protein, HEK (PROTP08254)
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