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Product Info Summary
| SKU: | M00110-3 |
|---|---|
| Size: | 20μL, 40μL, 100μL, 200μL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IP, IF, IHC, WB |
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Product info
Product Name
Anti-SP1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00110-3
Size
20μL, 40μL, 100μL, 200μL
Description
This Recombinant Rabbit Monoclonal Antibody detects endogenous levels of SP1 protein. Validated for research with WB,IHC,IF,IP,ELISA applications. Anti SP1 is reactive for Human samples
Storage & Handling
Store at -20°C/1 year
Cite This Product
Anti-SP1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00110-3)
Host
Rabbit
Contents
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% stabilizing protein
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Isotype
IgG, Kappa
Reactive Species
M00110-3 is reactive to SP1 in Human
Calculated molecular weight
80.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00110-3 is guaranteed for ELISA, IP, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:200-1:1000
WB 1:1000-1:5000
IF 1:200-1:1000
ELISA 1:5000-1:20000
IP 1:50-1:200
Validation Images & Assay Conditions
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Mouse colon was stained with Anti-SP1 rabbit antibody
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Rat colon was stained with Anti-SP1 rabbit antibody
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Human colon was stained with Anti-SP1 rabbit antibody
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Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SP1 antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Lane 1: Caco-2
Lane 2: HeLa treated with serum for 30 minutes
Predicted band size: 100kDa
Observed band size: 100kDa
Specific Publications For Anti-SP1 Rabbit Monoclonal Antibody (M00110-3)
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