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Product Info Summary
| SKU: | M01116-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-SPI1 Antibody
SKU/Catalog Number
M01116-1
Size
50 µl
Description
Boster Bio Anti-SPI1 Antibody (Catalog # M01116-1). Tested in WB, IHC application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-SPI1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01116-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1835CT291.10.52
Isotype
IgG1,k
Immunogen
This SPI1 antibody is generated from a mouse immunized with a recombinant protein of human SPI1.
Reactive Species
M01116-1 is reactive to SPI1 in Human
Calculated molecular weight
31.1 kDa
Background of SPI1
Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01116-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
Validation Images & Assay Conditions
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All lanes : Anti-SPI1 Antibody at 1:500-1:1000 dilution
Lane 1: K562 whole cell lysate
Lane 2: Daudi whole cell lysate
Lane 3: THP-1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 31 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-SPI1 Antibody (M01116-1)
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