Anti-SPIC Antibody

Spic antibody

Boster Bio Anti-SPIC Antibody (Catalog # A13433). Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A13433
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-SPIC Antibody

View all Spic Antibodies

SKU/Catalog Number

A13433

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-SPIC Antibody (Catalog # A13433). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SPIC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13433)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human SPIC.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A13433 is reactive to Spic in Human

Applications

A13433 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

27.942kDa

Background of Spic

Controls the development of red pulp macrophages required for red blood cells recycling and iron homeostasis. Transcription factor that binds to the PU-box, a purine-rich DNA sequence (5'-GAGGA[AT]-3') that can act as a lymphoid-specific enhancer. Regulates VCAM1 gene expression By similarity.

Carlsson R., Gene 299:271-278(2002).
The MGC Project Team, Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For Spic (Source: Uniprot.org, NCBI)

Gene Name

Spic

Full Name

Transcription factor Spi-C

Weight

27.942kDa

Superfamily

ETS family

Alternative Names

MGC40611; Spi-C transcription factor (Spi-1/PU.1 related); SPI-C; transcription factor Spi-C Spic|AU019198, C76795, P, Prf, Sp, Spi-C|Spi-C transcription factor (Spi-1/PU.1 related)|transcription factor Spi-C|pu.1-related factor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Spic, check out the Spic Infographic

Spic infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Spic: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-SPIC Antibody

Question

you antibody to test anti-SPIC antibody A13433 on human pancreas for research purposes, then I may be interested in using anti-SPIC antibody A13433 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

K. Krishna

Verified customer

Asked: 2020-02-18

Answer

The products we sell, including anti-SPIC antibody A13433, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-02-18

Question

Does A13433 anti-SPIC antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-06-28

Answer

As indicated on the product datasheet, A13433 anti-SPIC antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-06-28

Question

Is this A13433 anti-SPIC antibody reactive to the isotypes of SPIC?

Verified Customer

Verified customer

Asked: 2018-08-13

Answer

The immunogen of A13433 anti-SPIC antibody is Synthesized peptide derived from internal of human SPIC. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-08-13

Question

We are currently using anti-SPIC antibody A13433 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2018-05-07

Answer

The anti-SPIC antibody (A13433) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-05-07

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas using anti-SPIC antibody A13433. Let me know if you need anything else.

H. Rodriguez

Verified customer

Asked: 2018-02-15

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-02-15

Order DetailsPrice
A13433-100uL

100uL

$360

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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