Anti-Transcription factor Spi-B SPIB Antibody

Product Name

Anti-Transcription factor Spi-B SPIB Antibody

View all SPIB Antibodies

SKU/Catalog Number

A02805-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Transcription factor Spi-B SPIB Antibody (Catalog # A02805-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

SPIB antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Transcription factor Spi-B SPIB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02805-1)

Host

Rabbit

Contents

SPIB antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

SPIB antibody was raised against a 17 amino acid peptide near the carboxy terminus of human SPIB. The immunogen is located within amino acids 200 - 250 of SPIB.

Cross-reactivity

SPIB antibody is human, mouse and rat reactive. At least four isoforms of SPIB are known to exist; this antibody will detect all but isoform 3.

Reactive Species

A02805-1 is reactive to SPIB in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

28.8 kDa

Background of SPIB

SPIB is a member of the ETS transcription factor family that influences lymphoid development and activity and binds the consensus DNA site GGA (A/T) through a unique winged helix-turn-helix motif known as the ETS domain (1). SPIB is found in hematopoietic cells such as B cells and plasmacytoid dendritic cells (DC) (2,3). It promotes the development of plasmacytoid dendritic cells (pDCs) or natural interferon (IFN)-producing cells. SPIB may be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation (3,4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02805-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SPIB antibody can be used for detection of SPIB by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information SPIB

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