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Product Info Summary
| SKU: | A07281-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Spindlin-1 (H139) SPIN1 Antibody
SKU/Catalog Number
A07281-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Spindlin-1 (H139) SPIN1 Antibody catalog # A07281-1. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Spindlin-1 (H139) SPIN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07281-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human MY18B protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07281-1 is reactive to SPIN1 in Human, Mouse, Rat
Applications
A07281-1 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
29.601kDa
Background of SPIN1
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S12P family of ribosomal proteins. It is located in the cytoplasm. The protein shares significant amino acid similarity with S. cerevisiae ribosomal protein S28. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Hori N., Nucleic Acids Res. 21:4394-4394(1993).
Ebert L., Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Vladimirov S.N., Eur. J. Biochem. 239:144-149(1996).
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Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of SPIN1 using Anti-Spindlin-1 (H139) SPIN1 Antibody (A07281-1).
Immunohistochemistry (IHC) analyzes of Spindlin-1 (H139) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-Spindlin-1 (H139) SPIN1 Antibody A07281-1
Western blot (WB) analysis of Spindlin-1 (H139) pAb at 1:500 dilutionLane1:SK-OVCAR3 whole cell lysate(40ug)
Lane2:SGC7901 whole cell lysate(40ug)
Lane3:The Ovarian tissue lysate of Rat(40ug)
Lane4:The Testis tissue lysate of Mouse(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For SPIN1 (Source: Uniprot.org, NCBI)
Gene Name
SPIN1
Full Name
Spindlin-1
Weight
29.601kDa
Superfamily
SPIN/STSY family
Alternative Names
OCR; spindlin 1; spindlin; spindlin-1; SPINOvarian cancer-related protein SPIN1 SPIN, TDRD24 spindlin 1 spindlin-1|ovarian cancer-related protein|spindlin1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SPIN1, check out the SPIN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SPIN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Spindlin-1 (H139) SPIN1 Antibody (A07281-1)
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No publications found for A07281-1
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