Anti-SPOCK3 Antibody Picoband™

Product Name

Anti-SPOCK3 Antibody Picoband™

View all Testican 3/SPOCK3 Antibodies

SKU/Catalog Number

A03151-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-SPOCK3 Antibody Picoband™ catalog # A03151-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-SPOCK3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03151-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SPOCK3 recombinant protein (Position: Q104-H396).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03151-1 is reactive to SPOCK3 in Human

Applications

A03151-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

57 kDa

Calculated molecular weight

49.429kDa

Background of Testican 3/SPOCK3

This gene encodes a member of a novel family of calcium-binding proteoglycan proteins that contain thyroglobulin type-1 and Kazal-like domains. The encoded protein and may play a role in adult T-cell leukemia by inhibiting the activity of membrane-type matrix metalloproteinases. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of SPOCK3 using anti-SPOCK3 antibody (A03151-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPOCK3 antigen affinity purified polyclonal antibody (Catalog # A03151-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPOCK3 at approximately 57 kDa. The expected band size for SPOCK3 is at 57 kDa.

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Figure 2. IF analysis of SPOCK3 using anti-SPOCK3 antibody (A03151-1).
SPOCK3 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SPOCK3 Antibody (A03151-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For SPOCK3 (Source: Uniprot.org, NCBI)

Alternative Names

CWCV, and Kazal-like domains proteoglycan 3; HSAJ1454; sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 3; SPOCK3; TES-3; Testican 3; TICN3 SPOCK3 HSAJ1454, TES-3, TICN3 SPARC (osteonectin), cwcv and kazal like domains proteoglycan 3 testican-3|SPARC/osteonectin, cwcv and kazal like domains proteoglycan 3|sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 3

For more info on SPOCK3, check out the SPOCK3 Infographic

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In this infographic, you will see the following information for SPOCK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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