Click image to see more details
Product Info Summary
| SKU: | A03613 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Spred1 Antibody
SKU/Catalog Number
A03613
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Spred1 Antibody (Catalog # A03613). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Spred1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Spred1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03613)
Host
Rabbit
Contents
Spred1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Spred1 antibody was raised against a 20 amino acid synthetic peptide near the center of the human Spred1. The immunogen is located within amino acids 120 - 170 of Spred1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
IL-37 antibody is human specific. Multiple isoforms of IL-37 are known to exist; this antibody will only detect the largest isoform. IL-37 antibody is predicted to not cross-react with IL-36.
Reactive Species
A03613 is reactive to Spred1 in Human, Mouse, Rat
Applications
A03613 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
50.664kDa
Background of Spred1
The Ras-MAP kinase pathway is essential for the differentiation of neuronal cells and myocytes; it is inhibited by Spred1, a member of the Sprouty family of proteins. Spred1 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). This Spred1 antibody is predicted to have no cross-reactivity to Spred2 or Spred3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Spred1 antibody can be used for detection of Spred1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Spred1 in mouse brain tissue lysate with Spred1 antibody at (A) 1 and (B) 2 μg/mL.
Click image to see more details
Immunohistochemistry of Spred1 in human brain tissue with Spred1 antibody at 2.5 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For Spred1 (Source: Uniprot.org, NCBI)
Gene Name
Spred1
Full Name
Sprouty-related, EVH1 domain-containing protein 1
Weight
50.664kDa
Alternative Names
EVH1/Sprouty domain containing protein; FLJ33903; hSpred1; NFLS; SPRED1; spred-1; sprouty-related, EVH1 domain containing 1; sprouty-related, EVH1 domain-containing protein 1; suppressor of Ras/MAPK activation Spred1|5730461F13Rik, AL024345, AW047647, Spre|sprouty protein with EVH-1 domain 1, related sequence|sprouty-related, EVH1 domain-containing protein 1|sprouty-related protein 1 with EVH-1 domain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Spred1, check out the Spred1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Spred1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Spred1 Antibody (A03613)
Hello CJ!
No publications found for A03613
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Spred1 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Spred1 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
