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Product Info Summary
| SKU: | A16685 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-SPRYD4 Antibody
SKU/Catalog Number
A16685
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SPRYD4 Antibody (Catalog # A16685). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SPRYD4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SPRYD4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16685)
Host
Rabbit
Contents
SPRYD4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
SPRYD4 antibody was raised against an 18 amino acid synthetic peptide near the center of human SPRYD4. The immunogen is located within amino acids 70 - 120 of SPRYD4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
SPRYD4 antibody is predicted to not cross-react with other SPRYD protein family members. At least two isoforms of SPRYD4 are known to exist; this antibody will detect both isoforms.
Reactive Species
A16685 is reactive to Spryd4 in Human, Mouse, Rat
Applications
A16685 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
23.275kDa
Background of Spryd4
The SPRY domain-containing protein 4 (SPRYD4) is a member of a family of proteins whose sole common characteristic is the presence of a SPRY domain. SPRY domains are structural domains that were first described in the fungal Dictyostelium discoideum tyrosine kinase spore lysis A. In most systems SPRY domains provide binding sites for regulatory proteins or intramolecular binding sites that maintain the structural integrity of a protein. SPRYD4 is ubiquitously expressed and is most abundant in kidney, brain, bladder, thymus and stomach. Little is known of the function of the SPRYD4 protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SPRYD4 antibody can be used for detection of SPRYD4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SPRYD4 in fetal human liver tissue lysate with SPRYD4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
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Immunohistochemistry of SPRYD4 in human kidney tissue with SPRYD4 antibody at 2.5 μg/mL.
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Immunofluorescence of SPRYD4 in human kidney tissue with SPRYD4 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For Spryd4 (Source: Uniprot.org, NCBI)
Gene Name
Spryd4
Full Name
SPRY domain-containing protein 4
Weight
23.275kDa
Alternative Names
DKFZp686N0877; SPRY domain containing 4; SPRY domain-containing protein 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Spryd4, check out the Spryd4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Spryd4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SPRYD4 Antibody (A16685)
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No publications found for A16685
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Customer Reviews
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