Product Info Summary
SKU: | A08598 |
---|---|
Size: | 100μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-SR-1E HTR1E Antibody
SKU/Catalog Number
A08598
Size
100μl
Form
Liquid
Description
Boster Bio Anti-SR-1E HTR1E Antibody catalog # A08598. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SR-1E HTR1E Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08598)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
2G2
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human SR-1E. at AA rangle: 70-150
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A08598 is reactive to HTR1E in Human
Applications
A08598 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
41.682kDa
Background of 5-HT1E
APC11 is also known as Anaphase promoting complex subunit 11, APC11, Cyclosome subunit 11, Hepatocellular carcinoma associated RING finger protein, and HSPC214. APC11 is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC11 may function to recruit the E2 ubiquitin-conjugating enzymes to the complex. APC11 interacts with the cullin domain of ANAPC2 and also interacts with UBE2D2. APC11 shows both a cytoplasmic and nuclear localization. APC11 is expressed at high levels in skeletal muscle and heart; in moderate levels in brain, kidney, and liver; and at low levels in colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte. APC11 is a member of the RING-type zinc finger family and is auto-ubiquitinylated.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of specific cells using SR-1E Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For HTR1E (Source: Uniprot.org, NCBI)
Gene Name
HTR1E
Full Name
5-hydroxytryptamine receptor 1E
Weight
41.682kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
5HT1E; 5-HT1E; 5-HT-1E; 5-hydroxytryptamine (serotonin) receptor 1E; HTR1E; S31; S31,5-HT1E5-hydroxytryptamine receptor 1E; Serotonin receptor 1E HTR1E 5-HT1E 5-hydroxytryptamine receptor 1E 5-hydroxytryptamine receptor 1E|5-HT-1E|5-hydroxytryptamine (serotonin) receptor 1E, G protein-coupled|S31|serotonin receptor 1E
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HTR1E, check out the HTR1E Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HTR1E: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SR-1E HTR1E Antibody (A08598)
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No publications found for A08598
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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5 Customer Q&As for Anti-SR-1E HTR1E Antibody
Question
you antibody to test anti-SR-1E antibody A08598 on human brain for research purposes, then I may be interested in using anti-SR-1E antibody A08598 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
The products we sell, including anti-SR-1E antibody A08598, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-21
Question
My question regarding product A08598, anti-SR-1E antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-10-31
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08598 anti-SR-1E antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-31
Question
We are currently using anti-SR-1E antibody A08598 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-11
Answer
The anti-SR-1E antibody (A08598) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-11
Question
Here is the WB image, lot number and protocol we used for brain using anti-SR-1E antibody A08598. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-10-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-03
Question
Is this A08598 anti-SR-1E antibody reactive to the isotypes of HTR1E?
M. Anderson
Verified customer
Asked: 2017-06-05
Answer
The immunogen of A08598 anti-SR-1E antibody is Synthesized peptide derived from the Internal region of human SR-1E. at AA rangle: 70-150. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-06-05