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Product Info Summary
| SKU: | A00107 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC-P, WB |
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Product info
Product Name
Anti-SRC Antibody (N-term)
SKU/Catalog Number
A00107
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-SRC Antibody (N-term) (Catalog # A00107). Tested in WB, IF, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-SRC Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00107)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This SRC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SRC.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00107 is reactive to SRC in Human, Mouse
Calculated molecular weight
59.8 kDa
Background of SRC
The gene for SRC is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. SRC, the protein encoded by this gene, is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in SRC could be involved in the malignant progression of colon cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00107 is guaranteed for IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
IHC-P: 1:50-1:100
Validation Images & Assay Conditions
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Confocal immunofluorescent analysis of SRC Antibody (N-term) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
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Western blot analysis of lysates from MCF-7,rat PC-12,mouse C2C12 cell line (from left to right),using SRC Antibody (R14) (Cat. #A00107). A00107 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Specific Publications For Anti-SRC Antibody (N-term) (A00107)
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