Anti-SRD5A2 Rabbit Monoclonal Antibody

Product Name

Anti-SRD5A2 Rabbit Monoclonal Antibody

View all SRD5A2 Antibodies

SKU/Catalog Number

M00704

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-SRD5A2 Rabbit Monoclonal Antibody catalog # M00704. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SRD5A2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00704)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

24S47

Isotype

IgG

Immunogen

A synthesized peptide derived from human SRD5A2

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00704 is reactive to SRD5A2 in Human, Mouse, Rat

Applications

M00704 is guaranteed for IP, WB Boster Guarantee

Observed Molecular Weight

28 kDa

Calculated molecular weight

28.393kDa

Background of SRD5A2

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IP 1:50

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SRD5A2 using anti-SRD5A2 antibody (M00704).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: human NIH/3T3 whole cell lysates,
Lane 6: mouse HT22 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRD5A2 antigen affinity purified monoclonal antibody (Catalog # M00704) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRD5A2 at approximately 28 kDa. The expected band size for SRD5A2 is at 28 kDa.

Gene/Protein Information For SRD5A2 (Source: Uniprot.org, NCBI)

Alternative Names

3-oxo-5-alpha-steroid 4-dehydrogenase 2,5 alpha-SR2; EC 1.3.99.5; MGC138457; S5AR 2; SR type 2; Steroid 5-alpha-reductase 2,3-oxo-5 alpha-steroid 4-dehydrogenase 2; steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta4-dehydrogenase alpha 2); Type II 5-alpha reductase SRD5A2 steroid 5 alpha-reductase 2 3-oxo-5-alpha-steroid 4-dehydrogenase 2|5 alpha-SR2|S5AR 2|SR type 2|steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2)|type II 5-alpha reductase

For more info on SRD5A2, check out the SRD5A2 Infographic

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In this infographic, you will see the following information for SRD5A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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