Anti-ST2 Il1rl1 Antibody

Product Name

Anti-ST2 Il1rl1 Antibody

View all ST2/IL-33R Antibodies

SKU/Catalog Number

A00492

Size

100µg

Form

Liquid

Description

Boster Bio Anti-ST2 Il1rl1 Antibody (Catalog# A00492). Tested in IHC, WB application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ST2 Il1rl1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00492)

Host

Rabbit

Contents

Liquid. In PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

CFA-18

Isotype

Rabbit IgG

Immunogen

Synthetic peptide corresponding to the sequence near the N-terminus of mouse ST2. The sequence is common to all three ST2 isoforms

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Predicted species reactivity based on epitope sequence homology: mouse, gerbil.

Reactive Species

A00492 is reactive to IL1RL1 in Human, Mouse

Applications

A00492 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

63.358kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (2µg/ml)
Western Blot (1-2µg/ml). Detects a band of ~45kDa.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Il1rl1 using anti-Il1rl1 antibody (A00492).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Il1rl1 antigen affinity purified polyclonal antibody (Catalog # A00492) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Il1rl1.

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Figure 2. IHC analysis of Il1rl1 using anti-Il1rl1 antibody (A00492).
Il1rl1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Il1rl1 Antibody (A00492) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For IL1RL1 (Source: Uniprot.org, NCBI)

Alternative Names

DER4; DER4ST2growth stimulation-expressed; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33 R; IL33R; IL-33R; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; interleukin-1 receptor-like 1; Ly84; MGC32623; Protein ST2; ST2; ST2L; ST2V; T1; T1homolog of mouse growth stimulation-expressed IL1RL1 DER4, FIT-1, IL33R, ST2, ST2L, ST2V, T1 interleukin 1 receptor like 1 interleukin-1 receptor-like 1|growth stimulation-expressed|homolog of mouse growth stimulation-expressed|interleukin 1 receptor-related protein

For more info on IL1RL1, check out the IL1RL1 Infographic

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In this infographic, you will see the following information for IL1RL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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