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Product Info Summary
| SKU: | A03903-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SULF2 Antibody Picoband™
View all Sulfatase-2/SULF2 Antibodies
SKU/Catalog Number
A03903-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-SULF2 Antibody Picoband™ catalog # A03903-2. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SULF2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03903-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SULF2 recombinant protein (Position: K471-D614).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03903-2 is reactive to SULF2 in Human
Applications
A03903-2 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
135 kDa
Calculated molecular weight
100.455kDa
Background of Sulfatase-2/SULF2
Extracellular sulfatase Sulf-2 is an enzyme that in humans is encoded by the SULF2 gene. Heparan sulfate proteoglycans (HSPGs) act as coreceptors for numerous heparin-binding growth factors and cytokines and are involved in cell signaling. Heparan sulfate 6-O-endosulfatases, such as SULF2, selectively remove 6-O-sulfate groups from heparan sulfate. This activity modulates the effects of heparan sulfate by altering binding sites for signaling molecules
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SULF2 using anti-SULF2 antibody (A03903-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SULF2 antigen affinity purified polyclonal antibody (Catalog # A03903-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SULF2 at approximately 135 kDa. The expected band size for SULF2 is at 100,130-135 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SULF2 (Source: Uniprot.org, NCBI)
Gene Name
SULF2
Full Name
Extracellular sulfatase Sulf-2
Weight
100.455kDa
Superfamily
sulfatase family
Alternative Names
DKFZp313E091; EC 3.1.6; EC 3.1.6.-; EC 3.1.6.14; extracellular sulfatase Sulf-2; FLJ44488; FLJ90554; hSulf-2; KIAA1247HSULF-2; MGC126411; SULF2; SULF-2; sulfatase 2; Sulfatase2; Sulfatase-2 SULF2 HSULF-2 sulfatase 2 extracellular sulfatase Sulf-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SULF2, check out the SULF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SULF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SULF2 Antibody Picoband™ (A03903-2)
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No publications found for A03903-2
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Customer Reviews
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