Anti-SYBL1/VAMP7 Antibody Picoband™

Product Name

Anti-SYBL1/VAMP7 Antibody Picoband™

View all VAMP-7 Antibodies

SKU/Catalog Number

PB10017

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SYBL1/VAMP7 Antibody Picoband™ catalog # PB10017. Tested in WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SYBL1/VAMP7 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10017)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human SYBL1 recombinant protein (Position: N24-T190). Human SYBL1 shares 97.6% and 95.2% amino acid (aa) sequence identity with mouse and rat SYBL1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB10017 is reactive to VAMP7 in Human, Rat

Applications

PB10017 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

25 kDa

Calculated molecular weight

24.935kDa

Background of VAMP-7

Vesicle-associated membrane protein 7 (VAMP-7), also known as SYBL1, is a protein that in humans is encoded by the VAMP7 gene. This gene encodes a transmembrane protein that is a member of the soluble N-ethylmaleimide- sensitive factor attachment protein receptor (SNARE) family. The encoded protein localizes to late endosomes and lysosomes and is involved in the fusion of transport vesicles to their target membranes. Alternate splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SYBL1 using anti-SYBL1 antibody (PB10017).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYBL1 antigen affinity purified polyclonal antibody (Catalog # PB10017) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SYBL1 at approximately 25 kDa. The expected band size for SYBL1 is at 25 kDa.

Gene/Protein Information For VAMP7 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ54296; SYBL1; SYBL1FLJ53762; synaptobrevin-like 1; Synaptobrevin-like protein 1; tetanus neurotoxin-insensitive VAMP; Tetanus-insensitive VAMP; TIVAMP; TI-VAMP; VAMP7; VAMP-7; VAMP-7FLJ53045; vesicle-associated membrane protein 7 VAMP7 SYBL1, TI-VAMP, TIVAMP, VAMP-7 vesicle associated membrane protein 7 vesicle-associated membrane protein 7|synaptobrevin-like 1|synaptobrevin-like protein 1|tetanus neurotoxin-insensitive VAMP|tetanus-insensitive VAMP

For more info on VAMP7, check out the VAMP7 Infographic

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In this infographic, you will see the following information for VAMP7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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