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Product Info Summary
| SKU: | A03201 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-VAMP7 Antibody
SKU/Catalog Number
A03201
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-VAMP7 Antibody (Catalog # A03201). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
VAMP7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-VAMP7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03201)
Host
Rabbit
Contents
VAMP7 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
VAMP7 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human VAMP7. The immunogen is located within amino acids 20 - 70 of VAMP7.
Cross-reactivity
At least three isoforms of VAMP7 are known to exist; this antibody will detect the two longest isoforms. This antibody is predicted to not cross-react with other Ras-related proteins.
Reactive Species
A03201 is reactive to VAMP7 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
24.9 kDa
Background of VAMP7
VAMP7 is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) family, localizing to late endosomes and lysosomes. VAMP7 is thought to mediate the fusion of endosomes to their target lysosomes as well as other exocytosis events during phagocytosis and neuritogenesis. VAMP7 interacts with the VPS9 ankyrin repeat protein VARP, a protein that localizes to early endosomes and thought to regulate endosome dynamics. Together with CD82, VAMP7 can modulate the signaling of EGFR by regulating its endocytosis from the plasma membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03201 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
VAMP7 antibody can be used for detection of VAMP7 by Western blot at 0.5 - 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of VAMP7 in mouse lung tissue lysate with VAMP7 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunofluorescence of VAMP7 in human lung tissue with VAMP7 antibody at 20 μg/mL.
Specific Publications For Anti-VAMP7 Antibody (A03201)
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