Anti-SYK Purified Monoclonal Antibody

Product Name

Anti-SYK Purified Monoclonal Antibody

View all SYK Antibodies

SKU/Catalog Number

M00490

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SYK Purified Monoclonal Antibody (Catalog# M00490). Tested in IP, WB, IHC-P, ICC application(s). This antibody reacts with Human, Rat, Mouse.

Storage & Handling

Store at 2-8°C. Do not freeze.

Cite This Product

Anti-SYK Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00490)

Host

Mouse

Contents

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Clonality

Monoclonal

Clone Number

SYK-01

Isotype

Mouse IgG1

Immunogen

Recombinant fragment (aa 5-360) of human Syk. The antibody SYK-01 reacts with protein tyrosine kinase p72Syk (Syk; an intracellular antigen), which is required for the transduction of signals through the B cell antigen receptor (BCR) and the high affinity IgE receptor (FcepsilonRI).

Reactive Species

M00490 is reactive to SYK in Human, Mouse, Rat

Observed Molecular Weight

42 kDa

Calculated molecular weight

72.1 kDa

Background of SYK

Syk is a cytoplasmic protein tyrosine kinase that translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M00490 is guaranteed for IP, IHC-P, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1-2 μg/ml.
Immunohistochemistry (paraffin sections): 5 μg/ml; positive tissue: tonsil B cells.

Validation Images & Assay Conditions

See full target information SYK

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis