Product Info Summary
SKU: | M00490 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | IP, IHC-P, ICC, WB |
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Product info
Product Name
Anti-SYK Purified Monoclonal Antibody
SKU/Catalog Number
M00490
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SYK Purified Monoclonal Antibody (Catalog# M00490). Tested in IP, WB, IHC-P, ICC application(s). This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-SYK Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00490)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
SYK-01
Isotype
Mouse IgG1
Immunogen
Recombinant fragment (aa 5-360) of human Syk. The antibody SYK-01 reacts with protein tyrosine kinase p72Syk (Syk; an intracellular antigen), which is required for the transduction of signals through the B cell antigen receptor (BCR) and the high affinity IgE receptor (FcepsilonRI).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00490 is reactive to SYK in Human, Mouse, Rat
Applications
M00490 is guaranteed for IP, IHC-P, ICC, WB Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
72.066kDa
Background of SYK
Syk is a cytoplasmic protein tyrosine kinase that translocates to the plasma membrane upon B cell antigen receptor (BCR) or the high-affinity IgE receptor (FcepsilonRI) triggering, and phosphorylates downstream adaptor proteins, thereby providing docking sites for initiation of subsequent signaling pathways, such as calcium mobilization, cytoskeleton remodeling, or transcription of specific genes. Syk binds to the receptor assemblies through interactions of its pair of SH2 domains with ITAM motives of the receptor, which have been phosphorylated by Src-family kinases. These kinases also help to activate Syk by phosphorylation of its activation loop.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1-2 μg/ml.
Immunohistochemistry (paraffin sections): 5 μg/ml; positive tissue: tonsil B cells.
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of SYK using Anti-SYK Purified Monoclonal Antibody (M00490).
Immunocytochemistry analysis of Syk in human primary fibroblasts using anti-Syk (SYK-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).Click image to see more details
Figure 3. Immunocytochemistry staining of SYK using Anti-SYK Purified Monoclonal Antibody (M00490).
Immunocytochemistry analysis of Syk in human HeLa cell line using anti-Syk (SYK-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).Click image to see more details
Figure 1. Western blotting validation for Anti-SYK Purified Monoclonal Antibody M00490
Western blotting analysis (non-reducing conditions) of whole cell lysate of RAMOS human Burkitt lymphoma cell line (1)Protein Target Info & Infographic
Gene/Protein Information For SYK (Source: Uniprot.org, NCBI)
Gene Name
SYK
Full Name
Tyrosine-protein kinase SYK
Weight
72.066kDa
Superfamily
protein kinase superfamily
Alternative Names
DKFZp313N1010; EC 2.7.10; EC 2.7.10.2; FLJ25043; spleen tyrosine kinaseFLJ37489; SYK; tyrosine-protein kinase SYK SYK p72-Syk spleen associated tyrosine kinase tyrosine-protein kinase SYK|spleen tyrosine kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SYK, check out the SYK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SYK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SYK Purified Monoclonal Antibody (M00490)
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No publications found for M00490
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