Product Info Summary
SKU: | A02314-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, ICC, WB |
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Product info
Product Name
Anti-Synaptotagmin 1/SYT1 Antibody Picoband™
View all Synaptotagmin 1 Antibodies
SKU/Catalog Number
A02314-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Synaptotagmin 1/SYT1 Antibody Picoband™ catalog # A02314-1. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Synaptotagmin 1/SYT1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02314-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Synaptotagmin 1, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02314-1 is reactive to SYT1 in Human, Mouse, Rat
Applications
A02314-1 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
65 kDa
Calculated molecular weight
47.573kDa
Background of Synaptotagmin 1
Synaptotagmin-1 is a protein that in humans is encoded by the SYT1 gene. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca (2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Flow Cytometry, 1-3μg/1x106 cells
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Synaptotagmin 1 using anti-Synaptotagmin 1 antibody (A02314-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synaptotagmin 1 antigen affinity purified polyclonal antibody (Catalog # A02314-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Synaptotagmin 1 at approximately 65KD. The expected band size for Synaptotagmin 1 is at 47KD.
Click image to see more details
Figure 2. IHC analysis of Synaptotagmin 1 using anti-Synaptotagmin 1 antibody (A02314-1). Synaptotagmin 1 was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Synaptotagmin 1 Antibody (A02314-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Synaptotagmin 1 using anti-Synaptotagmin 1 antibody (A02314-1). Synaptotagmin 1 was detected in paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Synaptotagmin 1 Antibody (A02314-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Synaptotagmin 1 using anti-Synaptotagmin 1 antibody (A02314-1).
Synaptotagmin 1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Synaptotagmin 1 Antibody (A02314-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of Synaptotagmin 1 using anti-Synaptotagmin 1 antibody (A02314-1). Synaptotagmin 1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Synaptotagmin 1 Antibody (A02314-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. Flow Cytometry analysis of A549 cells using anti-Synaptotagmin 1 antibody (A02314-1).
Overlay histogram showing A549 cells stained with A02314-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Synaptotagmin 1 Antibody (A02314-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 7. Flow Cytometry analysis of PC-3 cells using anti-Synaptotagmin 1 antibody (A02314-1).
Overlay histogram showing PC-3 cells stained with A02314-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Synaptotagmin 1 Antibody (A02314-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 8. Flow Cytometry analysis of LLC cells using anti-Synaptotagmin 1 antibody (A02314-1).
Overlay histogram showing LLC cells stained with A02314-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Synaptotagmin 1 Antibody (A02314-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For SYT1 (Source: Uniprot.org, NCBI)
Gene Name
SYT1
Full Name
Synaptotagmin-1
Weight
47.573kDa
Superfamily
synaptotagmin family
Alternative Names
DKFZp781D2042; P65; SVP65; synaptotagmin Isynaptotagmin 1; Synaptotagmin1; Synaptotagmin-1; SYT; SYT1; sytI SYT1 BAGOS, P65, SVP65, SYT synaptotagmin 1 synaptotagmin-1|synaptotagmin I|sytI
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SYT1, check out the SYT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SYT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Synaptotagmin 1/SYT1 Antibody Picoband™ (A02314-1)
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Customer Q&As
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5 Customer Q&As for Anti-Synaptotagmin 1/SYT1 Antibody Picoband™
Question
We have seen staining in human pns. What should we do? Is anti-Synaptotagmin 1/SYT1 antibody supposed to stain pns positively?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
According to literature pns does express SYT1. According to Uniprot.org, SYT1 is expressed in middle temporal gyrus, amygdala brain, hippocampus, pns, among other tissues. Regarding which tissues have SYT1 expression, here are a few articles citing expression in various tissues:
Amygdala, and Brain, Pubmed ID: 14702039
Hippocampus, Pubmed ID: 17974005
PNS, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-11-06
Question
My lab would like using your anti-Synaptotagmin 1/SYT1 antibody for acetylcholine neurotransmitter release cycle studies. Has this antibody been tested with western blotting on a549 cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
I appreciate your inquiry. This A02314-1 anti-Synaptotagmin 1/SYT1 antibody is validated on rat brain tissue, mouse brain, human melanoma tissue, glioma tissue, a549 cells, llc cells. It is guaranteed to work for Flow Cytometry, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-17
Question
We are currently using anti-Synaptotagmin 1/SYT1 antibody A02314-1 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-26
Answer
The anti-Synaptotagmin 1/SYT1 antibody (A02314-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-26
Question
We ordered your anti-Synaptotagmin 1/SYT1 antibody for IHC on middle temporal gyrus in the past. I am using rat, and I plan to use the antibody for Flow Cytometry next. My lab would like examining middle temporal gyrus as well as amygdala brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for Flow Cytometry?
Verified Customer
Verified customer
Asked: 2018-05-15
Answer
I viewed the website and datasheets of our anti-Synaptotagmin 1/SYT1 antibody and it seems that A02314-1 has been tested on rat in both IHC and Flow Cytometry. Thus A02314-1 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-05-15
Question
Our team were satisfied with the WB result of your anti-Synaptotagmin 1/SYT1 antibody. However we have been able to see positive staining in hippocampus secretory vesicle using this antibody. Is that expected? Could you tell me where is SYT1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-07-20
Answer
According to literature, hippocampus does express SYT1. Generally SYT1 expresses in cytoplasmic vesicle, secretory vesicle. Regarding which tissues have SYT1 expression, here are a few articles citing expression in various tissues:
Amygdala, and Brain, Pubmed ID: 14702039
Hippocampus, Pubmed ID: 17974005
PNS, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-07-20