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Product Info Summary
| SKU: | PA2294 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SynCAM/CADM1 Antibody Picoband®
SKU/Catalog Number
PA2294
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SynCAM/CADM1 Antibody catalog # PA2294. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SynCAM/CADM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2294)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SynCAM, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2294 is reactive to CADM1 in Human, Mouse, Rat
Observed Molecular Weight
60 kDa
Calculated molecular weight
48.5 kDa
Background of CADM1
Cell adhesion molecule 1 is a protein that, in humans, is encoded by the CADM1 gene. This gene is also known as IGSF4, SYNCAM or TSLC1, and it is mapped to 11q23.3. CADM1 functions as a homophilic cell adhesion molecule at the synapse. Expression of the isolated cytoplasmic tail of CADM1 in neurons inhibited synapse assembly. Conversely, expression of full-length CADM1 in nonneuronal cells induced synapse formation by cocultured hippocampal neurons with normal release properties. CADM1 has been shown to interact with EPB41L3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2294 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: A549 Whole Cell, JURKAT Whole Cell, RAJI Whole Cell, HELA Whole Cell
Validation Images & Assay Conditions
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Anti-SynCAM antibody, PA2294, All Western blotting
All lanes: Anti-CADM1(PA2294) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: RAJI Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 60KD
Observed bind size: 60KD
Specific Publications For Anti-SynCAM/CADM1 Antibody Picoband® (PA2294)
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3 Customer Q&As for Anti-SynCAM/CADM1 Antibody Picoband®
Question
Is this PA2294 anti-SynCAM/CADM1 antibody reactive to the isotypes of CADM1?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
The immunogen of PA2294 anti-SynCAM/CADM1 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human SynCAM(73-92aa VIQLLNPNRQTIYFRDFRPL), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-13
Question
Can you help my question with product PA2294, anti-SynCAM/CADM1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2294 anti-SynCAM/CADM1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-13
Question
Here is the WB image, lot number and protocol we used for plasma {eco:0000269 using anti-SynCAM/CADM1 antibody PA2294. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-06
