Anti-TATA binding protein TBP Antibody Picoband™

Product Name

Anti-TATA binding protein TBP Antibody Picoband™

View all TATA binding protein TBP Antibodies

SKU/Catalog Number

A00302

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TATA binding protein TBP Antibody Picoband™ catalog # A00302. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TATA binding protein TBP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00302)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived mouse Tbp recombinant protein (Position: L38-A107).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00302 is reactive to TBP in Mouse, Rat

Applications

A00302 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

38 kDa

Calculated molecular weight

37.698kDa

Background of TATA binding protein TBP

TBP, TATA-binding protein, is a general transcription factor that binds specifically to a DNA sequence called the TATA box. The TBP gene is mapped to 6q27. This DNA sequence is found about 35 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitiation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Tbp using anti-Tbp antibody (A00302).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat testis tissue lysate,
Lane 2: rat ovary tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tbp antigen affinity purified polyclonal antibody (Catalog # A00302) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tbp at approximately 38KD. The expected band size for Tbp is at 38KD.

Gene/Protein Information For TBP (Source: Uniprot.org, NCBI)

Alternative Names

GTF2D; GTF2D1; HDL4; MGC126054; MGC126055; SCA17; TATA box binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-box binding protein N-terminal domain; TATA-box factor; TATA-box-binding protein; TF2D; TFIIDMGC117320; Transcription initiation factor TFIID TBP subunit TBP GTF2D, GTF2D1, HDL4, SCA17, TFIID TATA-box binding protein TATA-box-binding protein|TATA sequence-binding protein|TATA-box binding protein N-terminal domain|TATA-box factor|transcription initiation factor TFIID TBP subunit

For more info on TBP, check out the TBP Infographic

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In this infographic, you will see the following information for TBP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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