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Product Info Summary
| SKU: | A03371 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-TET3 Antibody
SKU/Catalog Number
A03371
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TET3 Antibody (Catalog # A03371). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
TET3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-TET3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03371)
Host
Rabbit
Contents
TET3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TET3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human TET3. The immunogen is located within amino acids 1520 - 1570 of TET3.
Cross-reactivity
At least three isoforms of TET3 are known to exist. TET3 antibody is predicted to not cross react with other TET proteins.
Reactive Species
A03371 is reactive to TET3 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
193.7 kDa
Background of TET3
TET3, a member of the ten-eleven-translocation (TET) family of genes, is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine and is most abundantly expressed in hematopoietic cells. Unlike the related TET2 protein, mutations in TET3 have not been observed in any myeloid malignancies. TET3 has been shown to be involved in the demethylation of zygotic DNA before the first mitosis and has been suggested to be involved in the epigenetic reprogramming of the zygotic paternal DNA following natural fertilization and may also contribute to somatic cell nuclear reprogramming during animal cloning.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03371 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TET3 antibody can be used for detection of TET3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TET3 in SK-N-SH cell lysate with TET3 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of TET3 in human brain tissue with TET3 antibody at 2.5 μg/ml.
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Immunofluorescence of TET3 in human brain tissue with TET3 antibody at 20 μg/mL.
Specific Publications For Anti-TET3 Antibody (A03371)
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