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Product Info Summary
| SKU: | A03761-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-TFIIIB90-1 Antibody
SKU/Catalog Number
A03761-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-TFIIIB90-1 Antibody catalog # A03761-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TFIIIB90-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03761-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
2F1
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human TF3B. AA range:231-280
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03761-1 is reactive to BRF1 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
73.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03761-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using TFIIIB90-1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of lysates from HeLa and Jurkat cells, using TF3B Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TF3B Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of MCF7 cells, using TF3B Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-TFIIIB90-1 Antibody (A03761-1)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-TFIIIB90-1 Antibody
Question
I see that the anti-TFIIIB90-1 antibody A03761-1 works with WB, what is the protocol used to produce the result images on the product page?
F. Singh
Verified customer
Asked: 2019-02-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-02-06
Question
Do you have a BSA free version of anti-TFIIIB90-1 antibody A03761-1 available?
V. Krishna
Verified customer
Asked: 2018-03-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TFIIIB90-1 antibody A03761-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-03-20
Question
Will A03761-1 anti-TFIIIB90-1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
N. Mitchell
Verified customer
Asked: 2017-12-15
Answer
You can see on the product datasheet, A03761-1 anti-TFIIIB90-1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-12-15
Question
Would anti-TFIIIB90-1 antibody A03761-1 work for WB with cervix carcinoma erythroleukemia?
B. Miller
Verified customer
Asked: 2013-02-07
Answer
According to the expression profile of cervix carcinoma erythroleukemia, BRF1 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-TFIIIB90-1 antibody A03761-1 will work for WB with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2013-02-07
