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Product Info Summary
| SKU: | A00019-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-TGF beta 1/TGFB1 Antibody
SKU/Catalog Number
A00019-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TGF beta 1/TGFB1 Antibody catalog # A00019-2. Tested in IHC applications. This antibody reacts with Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-TGF beta 1/TGFB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00019-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human TGF beta 1/TGFB1, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00019-2 is reactive to TGFB1 in Rat
Calculated molecular weight
44.3 kDa
Background of TGFB1
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00019-2 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Rat
Positive Control
IHC: rat liver tissue
Validation Images & Assay Conditions
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IHC analysis of TGF Beta 1/TGFB1 using anti-TGF Beta 1/TGFB1 antibody (A00019-2).
TGF Beta 1/TGFB1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TGF Beta 1/TGFB1 Antibody (A00019-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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The representative samples of immunohistochemical staining for AGE, RAGE, TGF-β1, TGF- β1 receptor, BDNF and TrkB in the colon wall of two groups. The microscopy with high magnification have been inserted in each single histological photo (arrow) in order to display the localization of markers. The staining of all proteins was stronger in the muscle layer than other layers. In the different layers, the staining of AGE, RAGE, TGF-β1 and TGF- β1 receptor was stronger whereas the staining of BDNF and TrkB was weaker in the Diabetes group than in Control group. Bar = 100 um.
Index in PubMed under a CC BY license. PMID: 29930382
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The fraction of AGE, RAGE, TGF-β1, TGF- β1 receptor, BDNF and TrkB in the different layers of the colon between two groups. In the different layers, the fraction of AGE, RAGE, TGF-β1 and TGF- β1 receptor was bigger whereas the fraction of BDNF and TrkB was smaller in the Diabetes group than in Control group. Compared with Control group: *P < 0.05, **P < 0.01.
Index in PubMed under a CC BY license. PMID: 29930382
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( A ) Correlation between AGE and RAGE in muscle layer and submucosa layer with circumferential constant a; ( B ) Correlation between AGE and RAGE in muscle layer and submucosa layer with longitudinal constant a; ( C ) Correlation between TGF-β1 and TGF-β1 receptor in mucosa layer and TGF-β1 muscle layer with circumferential and longitudinal material constant a; ( D ) Correlation between BDNF in muscle and submucosa layers with longitudinal constant a.
Index in PubMed under a CC BY license. PMID: 29930382
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( A ) Correlation between AGE and RAGE in different layers; ( B ) Correlation between TGF-β1 and TGF-β1receptor in different layers; ( C ) Correlation between BDNF and TrkB in different layers; ( D ) Correlation between RAGE and TGF-β1receptor in different layers.
Index in PubMed under a CC BY license. PMID: 29930382
Specific Publications For Anti-TGF beta 1/TGFB1 Antibody (A00019-2)
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