Anti-TGF beta 1/TGFB1 Antibody Picoband™

Product Name

Anti-TGF beta 1/TGFB1 Antibody Picoband™

View all TGFB1 Antibodies

SKU/Catalog Number

A00019-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TGF beta 1/TGFB1 Antibody Picoband™ catalog # A00019-2. Tested in IHC applications. This antibody reacts with Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-TGF beta 1/TGFB1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00019-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human TGF beta 1/TGFB1, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00019-2 is reactive to TGFB1 in Rat

Applications

A00019-2 is guaranteed for IHC Boster Guarantee

Observed Molecular Weight

120 kDa

Calculated molecular weight

44.341kDa

Background of TGFB1

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Rat

Validation Images & Assay Conditions

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Figure 1. IHC analysis of TGF Beta 1/TGFB1 using anti-TGF Beta 1/TGFB1 antibody (A00019-2).
TGF Beta 1/TGFB1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TGF Beta 1/TGFB1 Antibody (A00019-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For TGFB1 (Source: Uniprot.org, NCBI)

Alternative Names

CED; DPD1; LAP; TGFB; TGFB1; TGF-beta 1, 2, 3; TGFbeta; transforming growth factor, beta 1 TGFB1 CED, DPD1, IBDIMDE, LAP, TGF-beta1, TGFB, TGFbeta transforming growth factor beta 1 transforming growth factor beta-1 proprotein|TGF-beta-1|latency-associated peptide|prepro-transforming growth factor beta-1|transforming growth factor beta1

For more info on TGFB1, check out the TGFB1 Infographic

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In this infographic, you will see the following information for TGFB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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