Anti-TGFB1 Antibody

Product Name

Anti-TGFB1 Antibody

View all TGFB1 Antibodies

SKU/Catalog Number

A00019-1

Size

80 µl

Form

Liquid

Description

Boster Bio Anti-TGFB1 Antibody (Catalog # A00019-1). Tested in WB application(s). This antibody reacts with Human, Rat.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-TGFB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00019-1)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Clone Number

Clone HCGb/54 + HCGb/459

Isotype

Rabbit IgG

Immunogen

This TGFB1 antibody is generated from a rabbit immunized with a recombinant protein of human TGFB1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00019-1 is reactive to TGFB1 in Human, Rat

Applications

A00019-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

44.341kDa

Background of TGFB1

Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2000

Validation Images & Assay Conditions

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Western blot analysis of lysates from K562, L929 cell line and rat lung tissue (from left to right), using TGFB1 Antibody. A00019-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

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Anti-TGFB1 Antibody at 1:2000 dilution + A549 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 44 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Gene/Protein Information For TGFB1 (Source: Uniprot.org, NCBI)

Alternative Names

CED; DPD1; LAP; TGFB; TGFB1; TGF-beta 1, 2, 3; TGFbeta; transforming growth factor, beta 1 TGFB1 CED, DPD1, IBDIMDE, LAP, TGF-beta1, TGFB, TGFbeta transforming growth factor beta 1 transforming growth factor beta-1 proprotein|TGF-beta-1|latency-associated peptide|prepro-transforming growth factor beta-1|transforming growth factor beta1

For more info on TGFB1, check out the TGFB1 Infographic

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In this infographic, you will see the following information for TGFB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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