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Product Info Summary
| SKU: | A00019-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TGFB1 Antibody
SKU/Catalog Number
A00019-1
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-TGFB1 Antibody (Catalog # A00019-1). Tested in WB application(s). This antibody reacts with Human, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-TGFB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00019-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This TGFB1 antibody is generated from a rabbit immunized with a recombinant protein of human TGFB1.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00019-1 is reactive to TGFB1 in Human, Rat
Calculated molecular weight
44.3 kDa
Background of TGFB1
Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00019-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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Western blot analysis of lysates from K562, L929 cell line and rat lung tissue (from left to right), using TGFB1 Antibody. A00019-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
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Anti-TGFB1 Antibody at 1:2000 dilution + A549 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 44 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Specific Publications For Anti-TGFB1 Antibody (A00019-1)
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