Anti-TGF beta Receptor I/TGFBR1 Antibody Picoband™

Product Name

Anti-TGF beta Receptor I/TGFBR1 Antibody Picoband™

View all TGF-beta RI/ALK-5 Antibodies

SKU/Catalog Number

PB9828

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TGF beta Receptor I/TGFBR1 Antibody Picoband™ catalog # PB9828. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TGF beta Receptor I/TGFBR1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9828)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human TGFBR1, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9828 is reactive to TGFBR1 in Human

Applications

PB9828 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

50-56 kDa

Calculated molecular weight

55.96kDa

Background of TGF-beta RI/ALK-5

Transforming growth factor, beta receptor I is a TGF beta receptor. TGFBR1 is its human gene. The protein encoded by this gene forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF-beta signal from the cell surface to the cytoplasm. Mutations in this gene have been associated with Loeys-Dietz aortic aneurysm syndrome (LDAS). TGFB1 regulates cell cycle progression by a unique signaling mechanism that involves its binding to TGFBR2 and activation of TGFBR1. Both are transmembrane serine/threonine receptor kinases. The TGFBR1 receptor may be inactivated in many of the cases of human tumor cells refractory to TGFB-mediated cell cycle arrest. Vellucci and Reiss (1997) reported that the TGFBR1 gene is approximately 31 kb long and contains 9 exons. The organization of the segment of the gene that encodes the C-terminal portion of the serine/threonine kinase domain appears to be highly conserved among members of the gene family.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of TGFBR1/Tgf Beta Receptor I using anti-TGFBR1/Tgf Beta Receptor I antibody (PB9828).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SW579 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGFBR1/Tgf Beta Receptor I antigen affinity purified polyclonal antibody (Catalog # PB9828) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGFBR1/Tgf Beta Receptor I at approximately 50-56KD. The expected band size for TGFBR1/Tgf Beta Receptor I is at 56KD.

Gene/Protein Information For TGFBR1 (Source: Uniprot.org, NCBI)

Alternative Names

AAT5; activin A receptor type II-like kinase, 53kD; activin A receptor type II-like kinase, 53kDa; Activin receptor-like kinase 5; ACVRLK4; ALK-5; ALK-5ALK5; EC 2.7.11; EC 2.7.11.30; LDS1A; LDS2A; Serine/threonine-protein kinase receptor R4; SKR4; tbetaR-I; TGFB1R1; TGF-beta receptor type I; TGF-beta receptor type-1; TGF-beta RI; TGF-beta type I receptor; TGFbetaRI; TGFBR1; TGF-bRI; TGFR-1; transforming growth factor beta receptor I; transforming growth factor, beta receptor 1; transforming growth factor, beta receptor I (activin A receptor type II-likekinase, 53kD); Transforming growth factor TGFBR1 AAT5, ACVRLK4, ALK-5, ALK5, ESS1, LDS1, LDS1A, LDS2A, MSSE, SKR4, TBR-i, TBRI, TGFR-1, tbetaR-I transforming growth factor beta receptor 1 TGF-beta receptor type-1|activin A receptor type II-like kinase, 53kDa|activin A receptor type II-like protein kinase of 53kD|activin receptor-like kinase 5|mutant transforming growth factor beta receptor I|serine/threonine-protein kinase receptor R4|transforming growth factor beta receptor I|transforming growth factor-beta receptor type I

For more info on TGFBR1, check out the TGFBR1 Infographic

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In this infographic, you will see the following information for TGFBR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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