Anti-Thyroid hormone receptor alpha THRA Antibody

Product Name

Anti-Thyroid hormone receptor alpha THRA Antibody

View all TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibodies

SKU/Catalog Number

A01775

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Thyroid hormone receptor alpha THRA Antibody (Catalog # A01775). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Thyroid hormone receptor alpha THRA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01775)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human THRA isoform 1 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01775 is reactive to THRA in Human

Applications

A01775 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

54.816kDa

Background of TR alpha/NR1A1/Thyroid Hormone Receptor alpha

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Thyroid hormone receptor alpha is a nuclear hormone receptor with high affinity for the hormone triiodo-thyronine. THRA is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having a certain extent of redundancy, may mediate different functions of thyroid hormone. THRA interacts with NCOA3 and NCOA6 co-activators, leading to a strong increase in transcription of target genes. THRA is localized within the nucleus and has been found to exist as 4 isoforms originating from alternative splicing variants. This antibody recognizes THRA isoform 1. Isoform 1 has a distinct C-terminus compared to isoform 2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:300,000
IHC: 1:200
IF Microscopy: 1:200
IP: 1:100
WB: 1:500 - 1:2,000

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of THRA using anti-THRA antibody (A01775).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-THRA antigen affinity purified polyclonal antibody (Catalog # A01775) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for THRA.

Gene/Protein Information For THRA (Source: Uniprot.org, NCBI)

Alternative Names

AR7; c-ERBA-1; c-erbA-alpha; EAR7; EAR-7; EAR-7.1/EAR-7.2; ERBA; ERBA1thyroid hormone receptor, alpha (avian erythroblastic leukemia viral (v-erb-a)oncogene homolog); ERBA-related 7; MGC000261; MGC43240; NR1A 1; NR1A1; NR1A1THRA3; Nuclear receptor subfamily 1 group A member 1; THRA; THRA1ERB-T-1; THRA2; thyroid hormone receptor alpha; thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a)oncogene homolog, avian); TR alpha; triiodothyronine receptor; v-ErbA1; V-erbA-related protein 7 THRA AR7, CHNG6, EAR7, ERB-T-1, ERBA, ERBA1, NR1A11, THRA2, c-ERBA-1, THRA thyroid hormone receptor alpha thyroid hormone receptor alpha|EAR-7|ERBA-related 7|V-erbA-related protein 7|c-erbA-alpha|nuclear receptor subfamily 1 group A member 1|thyroid hormone receptor alpha 1|thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian)|thyroid normone nuclear receptor alpha variant 1|triiodothyronine receptor

For more info on THRA, check out the THRA Infographic

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In this infographic, you will see the following information for THRA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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