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- Table of Contents
TIMM17A · Western blot design guide
TIMM17A Western Blot Planning Guide
Plan a TIMM17A Western blot around the catalog-observed 18 kDa band, image-backed A12168-1 evidence, HPA controls, and verified protocol records.
Evidence assembled July 2026 · For research use; verify linked source records and product datasheet before use
TIMM17A Western Blot Experimental Design Guide
Expected bands, validated protocols, controls and antibodies — the at-a-glance facts below, then the full design guide.
Must know before running
| Expected band | 18 kDa | |
| Observed band | Not reported — verify product WB image | |
| Gel | 15% | |
| Positive control | Thyroid gland | |
| Negative control | Target knockdown/knockout |
Important caveats
Reasons your observed band may differ from the expected size.
| ⓘ | Calculated mass | 18 kDa |
| ⓘ | Localization | Mitochondrion inner membrane |
| ⓘ | Processing / PTM | Record-dependent |
| ⓘ | Reactivity | Human / Mouse / Rat |
Section 1
Real Curated TIMM17A Western Blot Protocols
Start with the molecular-weight rule, then compare verified publication-derived conditions.
| Sample / lysate | Esophagus |
| Gel % | 15% |
| Load | 20-30 µg total protein per lane |
| Transfer | Semi-dry, short transfer |
| Membrane | 0.2 µm PVDF |
| Blocking | 5% non-fat milk or 5% BSA in TBST |
| Primary | A12168-1 at datasheet starting dilution |
| Primary incubation | Overnight at 4 °C with gentle agitation |
| Secondary | Species-matched HRP conjugate at validated dilution |
| Wash | 3 × 5 min in TBST |
| Detection | Chemiluminescent substrate |
| Exposure | Bracket exposures to avoid saturation |
Section 2
What Is the Expected TIMM17A Western Blot Band Size?
Use the product-observed 18 kDa band as the primary planning value and retain the UniProt calculated mass as context.
| 18 kDa | Matches the authoritative product WB observation. |
| 18 kDa calculated | Use as UniProt context, not as a replacement observed band. |
| Unexpected additional signal | Do not assign identity without orthogonal positive/negative controls. |
💡Expected TIMM17A appearancePlan around 18 kDa and keep the calculated mass as supporting context.
| Catalog-observed band | 18 kDa; use this as the primary experimental expectation. |
| Calculated mass | 18 kDa from UniProt Q99595; retain as context. |
| Gel selection | 15%; shared with the recommended protocol and poster. |
| Specificity check | Compare the lead HPA positive and negative controls with A12168-1. |
| Situation | Likely cause | Next action |
|---|---|---|
| 18 kDa | Matches the authoritative product WB observation. | Confirm with orthogonal controls and the linked product record. |
| Additional band | May reflect processing, modification, or non-specific signal. | Run a dilution series and compare positive/negative controls. |
| Weak signal | Target abundance or transfer may be limiting. | Verify transfer, increase positive-control abundance, and bracket exposure. |
Experiment evidence
Western blot images showing TIMM17A band
A12168-1 · TIMM17A
← swipe / scroll for more images →
Real WB data behind the band-size expectations in this section.
Sample controls for TIMM17A Western blot
🧪Use Thyroid gland as the first positive-control candidate; no defensible HPA Not detected tissue was available, so use a target knockdown/knockout negative control.
Positive control: Thyroid gland (High)
Negative control: Target knockdown/knockout
HPA protein score determines control status; other expression data is supporting context only.
HPA tissue expression evidence for TIMM17A
Comprehensive Human Protein Atlas IHC scoring per tissue. Rows are taken directly from the HPA tissue chart — click any row's HPA link to view the source.
Positive expression · recommended positive controls
| Tissue | Cell type | Level | Evidence | Source |
|---|---|---|---|---|
| Thyroid gland | Reported tissue cells | High | Protein (HPA) | HPA → |
| Esophagus | Reported tissue cells | High | Protein (HPA) | HPA → |
| Heart muscle | Reported tissue cells | High | Protein (HPA) | HPA → |
| Oral mucosa | Reported tissue cells | High | Protein (HPA) | HPA → |
Undetected expression · recommended negative controls
| Tissue | Cell type | Level | Evidence | Source |
|---|
Section 3
Advanced TIMM17A Western Blot Tips
Deeper troubleshooting and optimisation questions for TIMM17A, answered from its protein features.
Which band should guide the blot?
Use 18 kDa, the observation attached to the authoritative A12168-1 WB record.
How should calculated mass be interpreted?
Treat the UniProt calculated mass as context; it does not replace the catalog-observed 18 kDa expectation.
Which positive control should I start with?
Start with Thyroid gland, the lead HPA protein-expression candidate.
Which negative control is defensible?
Use a target knockdown/knockout control when no HPA Not detected tissue is available.
Which gel should I use?
Use 15% consistently across the quick facts, protocol table, and poster.
What transfer method to use for TIMM17A Western blot?
Use the transfer method in the recommended protocol and verify transfer before blocking.
How should A12168-1 be started?
Start at the linked datasheet condition and run a three-point primary-antibody dilution test.
Which publication-derived protocols can I compare?
No verified publication protocol was supplied; use only the deterministic recommended protocol.
Boster reagents
TIMM17A Western Blot Reagents
Human/Mouse/Rat-reactive TIMM17A Western blot reagents with authoritative product imagery.
Real WB data
Anti-TIMM17A Antibody Picoband®
Cat # A12168-1
Only image-backed, WB-validated Human/Mouse/Rat recommendations from the prepared catalog evidence are shown.
Source: prepared picoband-wb product evidence; each card retains its own SKU, URL, observed band, and authoritative WB image.
References
- UniProt Q99595
- Human Protein Atlas — TIMM17A
- A12168-1 product record
- PMC6257833 — 'Governor vessel-unblocking and mind-regulating' acupuncture therapy ameliorates cognitive dysfunction in a rat model of middle cerebral artery occlusion (International journal of molecular medicine, 2019)
- PMC12132197 — HSF1 remodels mitochondrial biogenesis and function in cancer cells via TIMM17A (bioRxiv : the preprint server for biology, 2025)
- PMC8375323 — A TIMM17A Regulatory Network Contributing to Breast Cancer (Frontiers in genetics, 2021)