Anti-TIMP-3 (Tissue Inhibitor of Metalloproteinase-3) Antibody

TIMP-3 antibody

Boster Bio Anti-TIMP-3 (Tissue Inhibitor of Metalloproteinase-3) Antibody (Catalog # M00477). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Mouse, Rat, Horse, Cow, Dog. Cited in 3 publication(s).

Product Info Summary

SKU: M00477
Size: 100ug/vial
Reactive Species: Cow, Dog, Human, Mouse, Rat, Horse
Host: Rabbit
Application: Flow Cytometry, IF, IHC

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Product Name

Anti-TIMP-3 (Tissue Inhibitor of Metalloproteinase-3) Antibody

View all TIMP-3 Antibodies

SKU/Catalog Number

M00477

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-TIMP-3 (Tissue Inhibitor of Metalloproteinase-3) Antibody (Catalog # M00477). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Mouse, Rat, Horse, Cow, Dog.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-TIMP-3 (Tissue Inhibitor of Metalloproteinase-3) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00477)

Host

Rabbit

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Clonality

Polyclonal

Clone Number

Clone: GAL1/1831

Isotype

IgG

Immunogen

The amino acid sequence (aa175-211), used as the immunogen for TIMP3 Ab, is 100% homologous in human, cow, dog and horse, and 94% homologous in Mouse and rat.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00477 is reactive to TIMP3 in Cow, Dog, Human, Mouse, Rat, Horse

Applications

M00477 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

24.145kDa

Background of TIMP-3

TIMP3 (tissue inhibitor of metalloproteinases 3), along with family members TIMP1, TIMP2, and TIMP4, are inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix (ECM). An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. TIMP's inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. TIMP-3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Reconstitute with 1ml water (15 minutes at room temperature).

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For TIMP3 (Source: Uniprot.org, NCBI)

Gene Name

TIMP3

Full Name

Metalloproteinase inhibitor 3

Weight

24.145kDa

Superfamily

protease inhibitor I35 (TIMP) family

Alternative Names

HSMRK222; K222; K222TA2; metalloproteinase inhibitor 3; MIG-5 protein; Protein MIG-5; pseudoinflammatory); SFD; TIMP metallopeptidase inhibitor 3; TIMP3; TIMP-3; tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy; Tissue inhibitor of metalloproteinases 3 TIMP3 HSMRK222, K222, K222TA2, SFD TIMP metallopeptidase inhibitor 3 metalloproteinase inhibitor 3|MIG-5 protein|TIMP-3|protein MIG-5|tissue inhibitor of metalloproteinases 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TIMP3, check out the TIMP3 Infographic

TIMP3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TIMP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00477 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effect of Xianziyizhen Recipe Capsule on PGI2‐PPARδ Signaling Pathway in Embryo Implantation Dysfunction Mice

Promoter methylation and expression of TIMP3 gene in gastric cancer

Bai Yx, Yi Jl, Li Jf, Sui H. World J Gastroenterol. 2007 Jul 28;13(28):3883-5. Clinicopathologic Significance Of Bag1 And Timp3 Expression In Colon Carcinoma.

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6 Customer Q&As for Anti-TIMP-3 (Tissue Inhibitor of Metalloproteinase-3) Antibody

Question

Would anti-TIMP-3 (Tissue Inhibitor of Metalloproteinase-3) antibody M00477 work on zebrafish IHC with pancreas?

Verified Customer

Verified customer

Asked: 2020-04-03

Answer

Our lab technicians have not tested anti-TIMP-3 (Tissue Inhibitor of Metalloproteinase-3) antibody M00477 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-TIMP-3 (Tissue Inhibitor of Metalloproteinase-3) antibody M00477 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish pancreas in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-03

Question

Is a blocking peptide available for product anti-TIMP-3 (Tissue Inhibitor of Metalloproteinase-3) antibody (M00477)?

Verified Customer

Verified customer

Asked: 2019-12-13

Answer

We do provide the blocking peptide for product anti-TIMP-3 (Tissue Inhibitor of Metalloproteinase-3) antibody (M00477). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-12-13

Question

I was wanting to use your anti-TIMP-3 (Tissue Inhibitor of Metalloproteinase-3) antibody for IHC for bovine mammary carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for bovine mammary carcinoma identification?

Verified Customer

Verified customer

Asked: 2019-06-21

Answer

As indicated on the product datasheet, M00477 anti-TIMP-3 (Tissue Inhibitor of Metalloproteinase-3) antibody has been tested for IHC on bovine, canine, equine, human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in bovine mammary carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-21

Question

I am interested in to test anti-TIMP-3 (Tissue Inhibitor of Metalloproteinase-3) antibody M00477 on bovine mammary carcinoma for research purposes, then I may be interested in using anti-TIMP-3 (Tissue Inhibitor of Metalloproteinase-3) antibody M00477 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-02-06

Answer

The products we sell, including anti-TIMP-3 (Tissue Inhibitor of Metalloproteinase-3) antibody M00477, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-02-06

Question

Is there a BSA free version of anti-TIMP-3 (Tissue Inhibitor of Metalloproteinase-3) antibody M00477 available?

Verified Customer

Verified customer

Asked: 2017-11-29

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-TIMP-3 (Tissue Inhibitor of Metalloproteinase-3) antibody M00477 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-11-29

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mammary carcinoma using anti-TIMP-3 (Tissue Inhibitor of Metalloproteinase-3) antibody M00477. Let me know if you need anything else.

S. Collins

Verified customer

Asked: 2014-08-08

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-08-08

Order DetailsPrice
M00477-100ug

100 μg

$468

Free Secondary Antibody

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