Anti-TLE1 Rabbit Monoclonal Antibody

Product Name

Anti-TLE1 Rabbit Monoclonal Antibody

View all TLE1 Antibodies

SKU/Catalog Number

M02460

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-TLE1 Rabbit Monoclonal Antibody catalog # M02460. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TLE1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02460)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AFEA-20

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human TLE 1 Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02460 is reactive to TLE1 in Human, Mouse, Rat

Applications

M02460 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

50 kDa

Calculated molecular weight

83.201kDa

Background of TLE1

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

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Western blot analysis of TLE 1 expression in SH-SY5Y cell lysate.

Gene/Protein Information For TLE1 (Source: Uniprot.org, NCBI)

Alternative Names

E(Sp1) homolog; enhancer of split groucho 1; ESG; ESG1Enhancer of split groucho-like protein 1; GRG1; TLE1; transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila); transducin-like enhancer of split 1, homolog of Drosophila E(sp1); transducin-like enhancer protein 1 TLE1 ESG, ESG1, GRG1 TLE family member 1, transcriptional corepressor transducin-like enhancer protein 1|enhancer of split groucho-like protein 1|transducin like enhancer of split 1|transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila)

For more info on TLE1, check out the TLE1 Infographic

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In this infographic, you will see the following information for TLE1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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