Anti-TNF alpha Antibody Picoband™

TNF-alpha antibody

Boster Bio Anti-TNF alpha Antibody Picoband™ catalog # A00002-4. Tested in WB applications. This antibody reacts with Mouse. Cited in 10 publication(s).

Product Info Summary

SKU: A00002-4
Size: 100 μg/vial
Reactive Species: Mouse
Host: Rabbit
Application: WB

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Product Name

Anti-TNF alpha Antibody Picoband™

View all TNF-alpha Antibodies

SKU/Catalog Number



100 μg/vial




Boster Bio Anti-TNF alpha Antibody Picoband™ catalog # A00002-4. Tested in WB applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TNF alpha Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00002-4)




Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.




A synthetic peptide corresponding to a sequence of mouse TNF alpha (CNLPKYLDFAESGQVY).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00002-4 is reactive to Tnf in Mouse


A00002-4 is guaranteed for WB Boster Guarantee

Background of TNF-alpha

TNFα (Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. And this cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Moreover, this cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For Tnf (Source:, NCBI)

Uniprot ID


Gene ID


Gene Name


Full Name

Tumor necrosis factor




tumor necrosis factor family

Alternative Names

APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha Tnf|DI, DIF, TNF-, TNF-a, TNF-alpha, TNFSF2, TNFalpha, Tna, Tnfs, Tnfsf1a, Tnlg1f, Tnf|tumor necrosis factor|tumor necrosis factor|cachectin|tumor necrosis factor ligand 1f|tumor necrosis factor ligand superfamily member 2|tumor necrosis factor-alpha

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Tnf, check out the Tnf Infographic

Tnf infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Tnf: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

A00002-4 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Liao L,Huang L,Wei X,Yin L,Wei X,Li T.Bioinformatic and biochemical studies of formononetin against liver injure.Life Sci.2021 Feb 16:119229.doi:10.1016/j.lfs.2021.119229.Epub ahead of print.PMID:33607154.
Species: Mouse

Liao L,Huang L,Wei X,Yin L,Wei X,Li T.Bioinformatic and biochemical studies of formononetin against liver injure.Life Sci.2021 Feb 16:119229.doi:10.1016/j.lfs.2021.119229.Epub ahead of print.PMID:33607154.
Species: Mouse

Ye D,Hu Y,Zhu N,Gu W,Long G,Tao E,Fang M,Jiang M. Exploratory Investigation of Intestinal Structure and Function after Stroke in Mice. Mediators Inflamm.2021 Feb 15;2021:1315797.doi: 10.1155/2021/1315797.PMID:33642941;PMCID:PMC7902147.
Species: Mouse
A00002-4 usage in article: APP:WB, SAMPLE:INTESTINE TISSUE, DILUTION:1:400

Li Y,Zheng D,Shen D,Zhang X,Zhao X,Liao H.Protective Effects of Two Safflower Derived Compounds, Kaempferol and Hydroxysafflor Yellow A, on Hyperglycaemic Stress-Induced Podocyte Apoptosis via Modulating of Macrophage M1/M2 Polarization.J Immunol Res.2020
Species: Mouse
A00002-4 usage in article: APP: WB, SAMPLE:MACROPHAGES, DILUTION:1:500

Resveratrol attenuates hyperoxia%u2010induced oxidative stress, inflammation and fibrosis and suppresses Wnt/%u03B2%u2010catenin signalling in lungs of neonatal rats

Efficacy of acetylshikonin in preventing obesity and hepatic steatosis in db/db mice

Protective effects of baicalin on carbon tetrachloride induced liver injury by activating PPAR%u03B3 and inhibiting TGF%u03B21

Immunomodulatory properties of quercetin-3-O-%u03B1-L-rhamnopyranoside from Rapanea melanophloeos against influenza a virus

Neuroprotective effects of %u03B1-lipoic acid on long-term experimental autoimmune encephalomyelitis.

Polymeric micelles for potentiated antiulcer and anticancer activities of naringin

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5 Customer Q&As for Anti-TNF alpha Antibody Picoband™


I am interested in using your anti-TNF alpha antibody for positive regulation of interleukin-6 production studies. Has this antibody been tested with western blotting on liver tissue? We would like to see some validation images before ordering.

R. Mitchell

Verified customer

Asked: 2020-02-13


I appreciate your inquiry. This A00002-4 anti-TNF alpha antibody is validated on mouse thymus tissue, spleen tissue, liver tissue, lung tissue, kidney tissue. It is guaranteed to work for WB in mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-02-13


We are currently using anti-TNF alpha antibody A00002-4 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse. Is it possible that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-28


The anti-TNF alpha antibody (A00002-4) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-28


We have been able to see staining in mouse leukocyte. Do you have any suggestions? Is anti-TNF alpha antibody supposed to stain leukocyte positively?

Verified Customer

Verified customer

Asked: 2018-01-18


According to literature leukocyte does express TNF. According to, TNF is expressed in leukocyte, blood, prostatic carcinoma, among other tissues. Regarding which tissues have TNF expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Prostatic carcinoma, Pubmed ID: 8597870, 10205166

Boster Scientific Support

Answered: 2018-01-18


Does A00002-4 cross react with human samples?

Verified Customer

Verified customer

Asked: 2017-08-03


A00002-4 does not cross-react with human samples.

Boster Scientific Support

Answered: 2017-08-03


My team were happy with the WB result of your anti-TNF alpha antibody. However we have observed positive staining in blood cell membrane using this antibody. Is that expected? Could you tell me where is TNF supposed to be expressed?

J. Li

Verified customer

Asked: 2016-08-12


Based on literature, blood does express TNF. Generally TNF expresses in cell membrane. Regarding which tissues have TNF expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334
Prostatic carcinoma, Pubmed ID: 8597870, 10205166

Boster Scientific Support

Answered: 2016-08-12



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